LOCUS       sequence-141133-         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FlAsH-BRET reporter to monitor conformational changes in Arrestin3. 
            Insertion of FlAsH motif (CCPGCC) following amino acid residue 263 
            of Arrestin..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Lee MH, Appleton KM, Strungs EG, Kwon JY, Morinelli TA, Peterson YK,
            Laporte SA, Luttrell LM
  TITLE     The conformational signature of beta-arrestin2 predicts its 
            trafficking and signalling functions.
  JOURNAL   Nature. 2016 Mar 31;531(7596):665-8. doi: 10.1038/nature17154. Epub 
            2016 Mar 23.
  PUBMED    27007854
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature17154"; journalName: "Nature"; date: "2016-03-31- 
            31"; volume: "531"; issue: "7596"; pages: "665-8"
COMMENT     Sequence Label: Rlucrarr3-Flash5
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             853..870
                     /codon_start=1
                     /product="tetracysteine peptide that binds biarsenical 
                     labeling reagents"
                     /label=tetracysteine tag
                     /translation="CCPGCC"
ORIGIN
        1 ctccggcgag ctctcgagaa ttctcacgcg tctgcaggat atcaagcttg cgttaccgga
       61 tccatgggtg aaaaaccagg gaccagggtc ttcaagaagt cgagccctaa ctgcaagctc
      121 accgtgtact tgggcaagcg tgactttgtg gatcacttgg acaaagtgga tcctgtcgat
      181 ggtgtggtgc ttgtggatcc tgactacttg aaggaccgga aagtgtttgt gaccctcacc
      241 tgtgccttcc gctatggccg agaagacctg gatgtactgg gcctgtcttt ccgcaaagat
      301 ctgttcatcg ccacctacca ggccttcccc cccatgccca acccacctcg gccccccacc
      361 cgcctacagg accgactgct gaagaagttg ggccagcatg cccacccctt ttttttcaca
      421 ataccccaga atttgccttg ctccgtcaca ctgcagccag gaccggagga cacagggaag
      481 gcctgtggag tagactttga gattcgagcc ttctgtgcca aatctataga agaaaaaagc
      541 cacaaaagga actccgtgcg gcttatcatc agaaaggtac agtttgctcc tgagacaccc
      601 ggcccccagc catcagctga aaccacacgc cacttcctca tgtctgaccg gaggtccctg
      661 cacctagagg cttccctgga caaagagctg tactaccatg gggaacccct caatgtcaac
      721 gtccacgtca ccaacaattc tgccaagacc gtcaagaaga tcagagtgtc tgtgagacag
      781 tatgccgaca tttgcctctt cagcaccgcg cagtacaagt gtcctgtggc tcagcttgaa
      841 caagatgacc agtgttgtcc tggttgttgt gtgtctccca gttccacatt ctgcaa
//
