LOCUS       sequence-141148-         693 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Masutomi K, Yu EY, Khurts S, Ben-Porath I, Currier JL, Metz GB, 
            Brooks MW, Kaneko S, Murakami S, DeCaprio JA, Weinberg RA, Stewart 
            SA, Hahn WC
  TITLE     Telomerase maintains telomere structure in normal human cells.
  JOURNAL   Cell. 2003 Jul 25. 114(2):241-53.
  PUBMED    12887925
REFERENCE   2  (bases 1 to 693)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2003 
            Jul 25. 114(2):241-53."
COMMENT     Sequence Label: pMKO.1 puro p53 shRNA 1
FEATURES             Location/Qualifiers
     source          1..693
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        60..308
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     60..80
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     231..250
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(392..412)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 gccctcactc cttctctagg cgccggaatt gaagatctgt cgacaaggtc gggcaggaag
       61 agggcctatt tcccatgatt ccttcatatt tgcatatacg atacaaggct gttagagaga
      121 taattagaat taatttgact gtaaacacaa agatattagt acaaaatacg tgacgtagaa
      181 agtaataatt tcttgggtag tttgcagttt taaaattatg ttttaaaatg gactatcata
      241 tgcttaccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
      301 gaaacaccgg tgactccagt ggtaatctac ttcaagagag tagattacac tggagtcttt
      361 ttgaattcgc cagcacagtg gtcgaccctg tggaatgtgt gtcagttagg gtgtggaaag
      421 tccccaggct tcccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
      481 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
      541 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
      601 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
      661 gcctcggcct ctgagctatt ccagaagtag tga
//
