LOCUS       sequence-141154-         975 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Escherichia coli acetyl-CoA:oxalate CoA-transferase (yfdE) 
            with N-terminal His6 tag, His233>Gly mutant (H6EcYfdE-H233G).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Kappock
  TITLE     Additional E. coli YfdE/EvgA constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK652
FEATURES             Location/Qualifiers
     source          1..975
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             4..26
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             45..62
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             72..89
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 aattttgttt aactttaaga aggagatata ccatgggcag cagccatcat catcatcatc
       61 acagcagcgg cctggtgccg cgcggcagcc atatgacaaa taatgaaagc aaagggccgt
      121 ttgaaggctt attagttatc gatatgacac atgtccttaa tggacctttc ggaactcaac
      181 ttctttgtaa tatgggcgca agggtaatta aagttgagcc gccgggtcat ggtgatgata
      241 cccgcacatt tggtccctat gtggatggac agtcactcta ttacagtttt attaatcatg
      301 gcaaagagag tgtggttctt gatttaaaga atgatcacga taaaagtata tttataaata
      361 tgctcaaaca agctgatgta ttagctgaga attttcgccc aggtacaatg gaaaaactgg
      421 ggttttcatg ggaaacgctt caagaaatca acccgcgcct catatatgct tcatcgtcag
      481 gtttcggaca taccggtccg ctaaaagatg ctcctgccta cgataccatc attcaggcaa
      541 tgagcgggat aatgatggaa acaggatatc ctgatgctcc gccagtgcgc gttggtacat
      601 ctcttgcgga tctatgcggc ggtgtctatt tattcagcgg aatagtgagt gcactttatg
      661 gtcgcgaaaa gagccagaga ggggcgcatg tcgatatagc gatgtttgat gccacgctga
      721 gttttctgga gcatggtctg atggcatata tcgcaactgg gaagtcacca caacgtctgg
      781 gaaatcgcgg tccctacatg gcaccttttg atgttttcaa tactcaggat aagccgatta
      841 cgatttgttg tggtaatgac aagctttttt ctgcgttatg ccaggcactg gagcttacgg
      901 aactggttaa tgatccccga tttagcagca atattttacg cgtacaaaac caggctattc
      961 ttaaacaata tattg
//
