LOCUS       sequence-141155-        1043 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Escherichia coli acetyl-CoA:oxalate CoA-transferase (yfdE) 
            with N-terminal His6 tag, His233>Gly mutant (H6EcYfdE-H233G).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Kappock
  TITLE     Additional E. coli YfdE/EvgA constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1043)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK652
FEATURES             Location/Qualifiers
     source          1..1043
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(22..39)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tttgttagca gccggatctc agtggtggtg gtggtggtgc tcgagttatg atgagaactc
       61 ctggcgaatt tgttccccat gctggtcgag cgttgccgct cccggcataa catgcgggtc
      121 cgcgcagccg ctgattttta tcggattacc cggcatcatt attcccccgg cttcaatcaa
      181 catatttctc gcctgagttt gtggcaattt aatggcctca gccacactta ataacggcgc
      241 gacgggtaca ccaacttcat gtattctggc taaccaaact tcagctgcct gcgtttttaa
      301 cgtccgctca atatattgtt taagaatagc ctggttttgt acgcgtaaaa tattgctgct
      361 aaatcgggga tcattaacca gttccgtaag ctccagtgcc tggcataacg cagaaaaaag
      421 cttgtcatta ccacaacaaa tcgtaatcgg cttatcctga gtattgaaaa catcaaaagg
      481 tgccatgtag ggaccgcgat ttcccagacg ttgtggtgac ttcccagttg cgatatatgc
      541 catcagacca tgctccagaa aactcagcgt ggcatcaaac atcgctatat cgacatgcgc
      601 ccctctctgg ctcttttcgc gaccataaag tgcactcact attccgctga ataaatagac
      661 accgccgcat agatccgcaa gagatgtacc aacgcgcact ggcggagcat caggatatcc
      721 tgtttccatc attatcccgc tcattgcctg aatgatggta tcgtaggcag gagcatcttt
      781 tagcggaccg gtatgtccga aacctgacga tgaagcatat atgaggcgcg ggttgatttc
      841 ttgaagcgtt tcccatgaaa accccagttt ttccattgta cctgggcgaa aattctcagc
      901 taatacatca gcttgtttga gcatatttat aaatatactt ttatcgtgat cattctttaa
      961 atcaagaacc acactctctt tgccatgatt aataaaactg taatagagtg actgtccatc
     1021 cacataggga ccaaatgtgc ggg
//
