LOCUS       sequence-141156-         925 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pAAV vector to express H2B-GFP and oG (optimized Glycoprotein) in a 
            Cre-dependent manner.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Kim EJ, Jacobs MW, Ito-Cole T, Callaway EM
  TITLE     Improved Monosynaptic Neural Circuit Tracing Using Engineered Rabies
            Virus Glycoproteins.
  JOURNAL   Cell Rep. 2016 Apr 26;15(4):692-699. doi: 
            10.1016/j.celrep.2016.03.067. Epub 2016 Apr 14.
  PUBMED    27149846
REFERENCE   2  (bases 1 to 925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2016.03.067"; journalName: "Cell Rep"; date: 
            "2016-04-26- 26"; volume: "15"; issue: "4"; pages: "692-699"
COMMENT     Sequence Label: pAAV-Ef1a-DIO-H2B-GFP-2A-oG-WPRE-hGH
FEATURES             Location/Qualifiers
     source          1..925
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(37..70)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(121..154)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 tcgtgaggta ccggatcctc tagagtcgac tccggaataa cttcgtatag gatactttat
       61 acgaagttat gcagaatggt agctggattg tagctgctat tagcaatatg aaacctctta
      121 ataacttcgt atagcataca ttatacgaag ttatggcgcg ccttagagcc gtgtctcgcc
      181 cccagattta tgactttccc aactgctgat gattttaccg ctctggggtg tgacagacac
      241 ttctctaccg gtccccctca gattgtgctg agttggctcg gagcggttca ctcgtcgcca
      301 gcaggtcatc aggaaaatga tcagcatgag agcagtcagt gctccagcgg agagcagcac
      361 gtacttgccc caattaggca gtccgagatc gacgccactg atccgttcgt gcacgtcggg
      421 caggtggacc tccacaaaat cttcggcctc gtcgccgttt ttgaacactg tgcttgggtc
      481 tgcgagagga tgcatcaggg gaatgacgct tgacacgagc agttccatgt gctgctggag
      541 cagactagac tgcatctcgg ggatcagcac gttaccatct ggccccagaa tgataccatt
      601 aaagaagacc ccgttcacat gtgggtgaca tcgacccccg actcgcaggc atcccttact
      661 aggaatgatt tcattccagg ttctcacaga tttataatgg gcgtctgcct ccatcagtgt
      721 cttgttaaag atggtgtatg cttttccgaa gccagggacg agcttcctca ggtggctcag
      781 cctcctgaag ctcacgctct tggtagtcat aattgactcc agagcatcga gacattcctc
      841 ccttttcttc acgagttcct cgaccaccag atgttcgatc tcatcgcttc ggaagtcgtg
      901 cagattcacg agctgcccag ggggg
//
