LOCUS       sequence-141164-         977 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses wildtype IDH1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Zhao S, Lin Y, Xu W, Jiang W, Zha Z, Wang P, Yu W, Li Z, Gong L, 
            Peng Y, Ding J, Lei Q, Guan KL, Xiong Y
  TITLE     Glioma-derived mutations in IDH1 dominantly inhibit IDH1 catalytic 
            activity and induce HIF-1alpha.
  JOURNAL   Science. 2009 Apr 10;324(5924):261-5. doi: 10.1126/science.1170944.
  PUBMED    19359588
REFERENCE   2  (bases 1 to 977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2009-04-10- 10"; volume: "324"; 
            issue: "5924"; pages: "261-5"
COMMENT     Sequence Label: pcDNA3-Flag-IDH1
FEATURES             Location/Qualifiers
     source          1..977
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     51..70
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        51..69
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tagcgactct ctggctactg agaacccact gcttactggc ttatcgaaat taatacgact
       61 cactataggg agacccaagc tggctagcgt ttaaacgggc cctctagact cgagcggccg
      121 ccactgtgct ggatatctgc agaattcatg tccaaaaaaa tcagtggcgg ttctgtggta
      181 gagatgcaag gagatgaaat gacacgaatc atttgggaat tgattaaaga gaaactcatt
      241 tttccctacg tggaattgga tctacatagc tatgatttag gcatagagaa tcgtgatgcc
      301 accaacgacc aagtcaccaa ggatgccgca gaagctataa agaagcataa tgttggcgtc
      361 aaatgtgcca ctatcactcc tgatgagaag agggttgagg agttcaagtt gaaacaaatg
      421 tggaaatcac caaatggcac catacgaaat attctgggtg gcacggtctt cagagaagcc
      481 attatctgca aaaatatccc ccggcttgtg agtggatggg taaaacctat catcataggt
      541 cgtcatgctt atggggatca atacagagca actgattttg ttgttcctgg gcctggaaaa
      601 gtagagataa cctacacacc aagtgacgga acccaaaagg tgacatacct ggtacataac
      661 tttgaagaag gtggtggtgt tgccatgggg atgtataatc aagataagtc aattgaagat
      721 tttgcacaca gttccttcca aatggctctg tctaagggtt ggcctttgta tctgagcacc
      781 aaaaacacta ttctgaagaa atatgatggg cgttttaaag acatctttca ggagatatat
      841 gacaagcagt acaagtccca gtttgaagct caaaagatct ggtatgagca taggctcatc
      901 gacgacatgg tggcccaagc tatgaaatca gagggaggct tcatctgggc ctgtaaaaac
      961 tatgatggtg acgtgca
//
