LOCUS       sequence-141166-         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses wildtype IDH1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Zhao S, Lin Y, Xu W, Jiang W, Zha Z, Wang P, Yu W, Li Z, Gong L, 
            Peng Y, Ding J, Lei Q, Guan KL, Xiong Y
  TITLE     Glioma-derived mutations in IDH1 dominantly inhibit IDH1 catalytic 
            activity and induce HIF-1alpha.
  JOURNAL   Science. 2009 Apr 10;324(5924):261-5. doi: 10.1126/science.1170944.
  PUBMED    19359588
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2009-04-10- 10"; volume: "324"; 
            issue: "5924"; pages: "261-5"
COMMENT     Sequence Label: pcDNA3-Flag-IDH1
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(12..29)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(45..74)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(88..111)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cgtttaactc aatgatgatg atgatgatgg tcgacggcgc tattcagatc ctcttctgag
       61 atgagttttt gttctagaaa gcttctactt atcgtcgtca tccttgtaat cgggatccac
      121 aagtttggcc tgagctagtt tgatcttcaa gttttctcca agtttatcca tgaactcaaa
      181 tgtattcaag tagtcagaac gttgcacatt gggtaaacct ttaatgcaag cagccaagtc
      241 cttggtcatg aagccagcct caattgtctc aatagagact tcttccaaag catttgcaaa
      301 gaaggcaagc tctttattgt tatcaagctt tgctctgtgg gctaaccctc tggtccaggc
      361 aaaaatggaa gcaatgggat tggtggacgt ctcctgtcct ttctggtaca tgcggtagtg
      421 tcgggttaca gtcccgtggg cagcctctgc ttctactgtc ttgccatctg gacaaaccag
      481 cacgctggtc atcatgccga gagagccata cccttgggcc acagagtccg actgcacgtc
      541 accatcatag tttttacagg cccagatgaa gcctccctct gatttcatag cttgggccac
      601 catgtcgtcg atgagcctat gctcatacca gatcttttga gcttcaaact gggacttgta
      661 ctgcttgtca tatatctcct gaaagatgtc tttaaaacgc ccatcatatt tcttcagaat
      721 agtgtttttg gtgctcagat acaaaggcca acccttagac agagccattt ggaaggaact
      781 gtgtgcaaaa tcttcaattg acttatcttg attatacatc cccatggcaa caccaccacc
      841 ttcttcaaag ttatgtac
//
