LOCUS       sequence-141167-         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  RBS_L2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pBP-TL9
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     5..22
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(46..103)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     terminator      complement(131..158)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(174..245)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(301..321)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 gcgcagcgag tcagtgagcg aggaagcctg cataacgcga agtaatcttt tcggttttaa
       61 agaaaaaggg cagggtggtg acaccttgcc cttttttgcc ggactgcagc gtctctggca
      121 aacatataaa cgcagaaagg cccacccgaa ggtgagccag tgtgactcta gtagagagcg
      181 ttcaccgaca aacaacagat aaaacgaaag gcccagtctt tcgactgagc ctttcgtttt
      241 atttgatgcc tggtcgatta tttgtatagt tcatccatgc catgtgtaat cccagcagct
      301 gttacaaact caagaaggac catgtgatct ctcttttcgt tgggatcttt cgaaagggca
      361 gattgtgtgg acaggtaatg gttgtctggt aaaaggacag ggccatcgcc aattggagta
      421 ttttgttgat aatggtctgc tagttgaacg cttccatctt caatgttgtg tctaattttg
      481 aagttaactt tgattccatt cttttgtttg tctgccatga tgtatacatt gtgtgagtta
      541 tagttgtatt ccaatttgtg tccaagaatg tttccatctt ctttaaaatc aatacctttt
      601 aactcgattc tattaacaag ggtatcacct tcaaacttga cttcagcacg tgtcttgtag
      661 ttcccgtcat ctttgaaaaa tatagttctt tcctgtacat aaccttcggg catggcactc
      721 ttgaaaaagt catgctgttt catatgatct gggtatctcg caaagcattg aacaccataa
      781 ccgaaagtag tgacaagtgt tggccatgga acaggtagtt ttccagtagt gcaaataaat
      841 ttaagg
//
