LOCUS       sequence-141174-         817 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PET RBS - derived from pET vectors (5' GTAC/3' CATA fusion).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pBP-pET-RBS
FEATURES             Location/Qualifiers
     source          1..817
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(41..98)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    182..201
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     terminator      204..247
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(251..269)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(497..516)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 gcgagtcagt gagcgaggaa gcctgcataa cgcgaagtaa tcttttcggt tttaaagaaa
       61 aagggcaggg tggtgacacc ttgccctttt ttgccggact gcagctaatg agacggcatg
      121 ggtctcttat gtatatctcc ttcttaaagt taaagtactg agacctatgc gtctctagat
      181 tctagaagcg gccgcgaatt ccagaaatca tccttagcga aagctaagga ttttttttat
      241 ctgaaattct gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
      301 tcttagacgt caggtggcac tcgagttgat cgggcacgta agaggttcca actttcacca
      361 taatgaaata agatcactac cgggcgtatt ttttgagtta tcgagatttt caggagctaa
      421 ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca
      481 tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt
      541 tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc
      601 ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaatttc gtatggcaat
      661 gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga
      721 gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct
      781 acacatatat tcgcaagatg tggcgtgtta cggtgaa
//
