LOCUS       sequence-141178-         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bba_B0034 iGEM RBS (5' GTAC/3' CATA fusion).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pBP_BBa_B0034
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     4..21
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(45..102)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     RBS             139..150
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     misc_feature    180..199
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     terminator      202..245
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(249..267)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(495..514)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 cgcagcgagt cagtgagcga ggaagcctgc ataacgcgaa gtaatctttt cggttttaaa
       61 gaaaaagggc agggtggtga caccttgccc ttttttgccg gactgcagct aatgagacgg
      121 catgggtctc ttatggtatt tctcctcttt ctgtactgag acctatgcgt ctctagattc
      181 tagaagcggc cgcgaattcc agaaatcatc cttagcgaaa gctaaggatt ttttttatct
      241 gaaattctgc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
      301 ttagacgtca ggtggcactc gagttgatcg ggcacgtaag aggttccaac tttcaccata
      361 atgaaataag atcactaccg ggcgtatttt ttgagttatc gagattttca ggagctaagg
      421 aagctaaaat ggagaaaaaa atcactggat ataccaccgt tgatatatcc caatggcatc
      481 gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg tacctataac cagaccgttc
      541 agctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag ttttatccgg
      601 cctttattca cattcttgcc cgcctgatga atgctcatcc ggaatttcgt atggcaatga
      661 aagacggtga gctggtgata tgggatagtg ttcacccttg ttacaccgtt ttccatgagc
      721 aaactgaaac gttttcatcg ctctggagtg aataccacga cgatttccgg cagtttctac
      781 acatatattc gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc cctaaagg
//
