LOCUS       sequence-141180-         835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  T7 promoter (5' CTAT/3' GTAC fusion).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pBP-T7
FEATURES             Location/Qualifiers
     source          1..835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     2..19
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(43..100)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(137..156)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(138..156)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    184..203
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     terminator      206..249
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(253..271)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(499..518)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 cagcgagtca gtgagcgagg aagcctgcat aacgcgaagt aatcttttcg gttttaaaga
       61 aaaagggcag ggtggtgaca ccttgccctt ttttgccgga ctgcagctaa tgagacggca
      121 tgggtctctg tactctccct atagtgagtc gtattaatag tgagacctat gcgtctctag
      181 attctagaag cggccgcgaa ttccagaaat catccttagc gaaagctaag gatttttttt
      241 atctgaaatt ctgcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
      301 tttcttagac gtcaggtggc actcgagttg atcgggcacg taagaggttc caactttcac
      361 cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt ttcaggagct
      421 aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg
      481 catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc
      541 gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat
      601 ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt tcgtatggca
      661 atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat
      721 gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt
      781 ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttcc
//
