LOCUS       sequence-141181-         953 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-dependent expression of Magneto2.0 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Wheeler MA, Smith CJ, Ottolini M, Barker BS, Purohit AM, Grippo RM, 
            Gaykema RP, Spano AJ, Beenhakker MP, Kucenas S, Patel MK, Deppmann 
            CD, Guler AD
  TITLE     Genetically targeted magnetic control of the nervous system.
  JOURNAL   Nat Neurosci. 2016 Mar 7. doi: 10.1038/nn.4265.
  PUBMED    26950006
REFERENCE   2  (bases 1 to 953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2016 Mar 7. doi: 10.1038/nn.4265."
COMMENT     Sequence Label: pAAV-CMV-DIO-Magneto2.0-sNRPpA
FEATURES             Location/Qualifiers
     source          1..953
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(58..91)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(142..175)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(280..303)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(898..921)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gctaactaga gaacccactg cttactggct tatcgaaatt ctagagtcga ctccggaata
       61 acttcgtata ggatacttta tacgaagtta tgcagaatgg tagctggatt gtagctgcta
      121 ttagcaatat gaaacctctt aataacttcg tatagcatac attatacgaa gttatggcgc
      181 gccggatccc acgttgatgt cgatctggtc cagggggatg tactcgccct cgctggtgat
      241 tctgctcttg ctagccactc gggaggagcc tccaccccct ttgtcatcgt cgtctttata
      301 gtcgctcccg ccacccccgc gtgcgctttc attatcactg tctcccaggg tgtgcttgtc
      361 aaagagatat tccgccaagc cagattcggg cgctcccatc ttgcgcaagt tggtcacgtg
      421 gtcacccaat tctttgatgg ctttcacctg ctcattcagg taatgtgtct caatgaagtc
      481 acacaaatgg gggtcatttt tgtcagtggc cagtttgtgc agttccagta gtgactgatt
      541 cacatttttt tccaaatgta atgcacactc cattgcattc agcccgctct cccagtcatc
      601 acagtctggt ttcttgatat cctgaaggaa gattcggcca cctcgttggt tctgcagctt
      661 catcagtttc tcagcatgtt ccctctcctc atgagattgg tgaagaaagt atttggcaaa
      721 gttcttcaaa gccacatcat cgcggtcaaa gtagtaagac atggacaggt aaacgtagga
      781 ggcgtagagc tccaggttga tctggcggtt gatggcggcc tctgagtcct ggtggtagtt
      841 ctggcgcacc tgcgaggtgg acgcggtcgt catgacgcgg gaggagccgc cgccgccttt
      901 atcatcatca tctttataat cggagccgcc gccgccgcgg gcgtcgtgct tga
//
