LOCUS       sequence-141182-         963 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide 
            synthetase glutaminase subunit (AaPurQ).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Kappock
  TITLE     A multisubunit Gram-negative FGAM synthetase
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK545
FEATURES             Location/Qualifiers
     source          1..963
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        57..75
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    76..100
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             115..137
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             156..173
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        287..305
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    306..330
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 gccggccacg atgcgtccgg cgtagaggat cgagatcgat ctcgatcccg cgaaattaat
       61 acgactcact ataggggaat tgtgagcgga taacaattcc cctctagaaa taattttgtt
      121 taactttaag aaggagatat accatgggca gcagccatca ccatcatcac cacagccagg
      181 atccgaattc gagctcggcg cgcctgcagg tcgacaagct tgcggccgca taatgcttaa
      241 gtcgaacaga aagtaatcgt attgtacacg gccgcataat cgaaattaat acgactcact
      301 ataggggaat tgtgagcgga taacaattcc ccatcttagt atattagtta agtataagaa
      361 ggagatatac atatgaaagc agcaattatc gtattccccg gcacaaaccg ggagcgcgat
      421 atggccattg ccctcaagcg cgttacaggg caggccccgc gcatgatctg gcaccatgaa
      481 acagatctgg gagataccga tctagtcgtg ctgccaggtg gtttcagcta tggcgattat
      541 ctgcgctgtg gtgccatggc agcgcattct cccatcatgg cggctatcaa agcctttgcc
      601 caaaagggtg gccatatttt gggtgtgtgc aacggcttcc agattttaac ggaaacaggg
      661 cttctgcccg gtgctctgtt gcgcaacgcc aacctgcgct ttctctctca ggattgctac
      721 ctgaaggtgg aagcagagaa cacacctttc actcacaagt ggaagaaggg tgatgttttc
      781 cgcacccctc tggcccatgg tgatggcaat tacacggcag atatcgccac gctggaacgg
      841 cttgaaggtg aaggtcttgt ggctttccac tatgccgatg ccaaaggcaa tgtgcaggct
      901 gatgatgcca tcagcaaccc caatggcagc atcaacagca ttgcgggtat tctgagcgaa
      961 aac
//
