LOCUS       sequence-141184-         726 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tissue-specific knock-out in zebrafish, Cas9 and GFP driven by a UAS
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Di Donato V, De Santis F, Auer TO, Testa N, Sanchez-Iranzo H, 
            Mercader N, Concordet JP, Del Bene F
  TITLE     2C-Cas9: a versatile tool for clonal analysis of gene function.
  JOURNAL   Genome Res. 2016 Mar 8.
  PUBMED    26957310
REFERENCE   2  (bases 1 to 726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome Res.
            2016 Mar 8."
COMMENT     Sequence Label: pUAS:Cas9T2AGFP;U6:sgRNA1;U6:sgRNA2
FEATURES             Location/Qualifiers
     source          1..726
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(59..78)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     113..132
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 agtaatctag aactatagtg agtcgtatta cgtagatcca gacatgataa gatacattga
       61 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
      121 tgatgctatt gctttatttg taaccattat aagctgcaat aaaccttgta caaagtgggg
      181 gatcctacgc ttacagctcc aaaactctac ggtaaaccta cataaactgc tggttttcaa
      241 attttaaaga atttaagggt ttacaggttt actactacac agtgatttac tgacacatgt
      301 aagtgtaaat gagttgaata agtaagtaag ccatatacca cacatgaaac acatacccag
      361 aagtcactgg tatatatagc cgtcctccag actcccagct cgagacgatt agatctaatg
      421 cgtctcgttt aagagctatg ctggaaacag catagcaagt ttaaataagg ctagtccgtt
      481 atcaacttga aaaagtggca ccgagtcggt gctttttttc aatgatgaat taattcgagc
      541 tccaccgcgg aagcttaaac aagaatctct agttttcttt cttgctttta cttttacttc
      601 cttaatactc aagtacaatt ttaatggagt acttttttac ttttactcaa gtaagattct
      661 agccagatac ttttactttt aattgagtaa aattttccct aagtacttgt actttcactt
      721 gagtaa
//
