LOCUS       sequence-141185-         982 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide 
            synthetase glutaminase subunit (AaPurQ).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Kappock
  TITLE     A multisubunit Gram-negative FGAM synthetase
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 982)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK545
FEATURES             Location/Qualifiers
     source          1..982
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(6..50)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     protein_bind    876..900
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(900..919)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(901..919)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gtagacgagt ccatgtgctg gcgttcaaat ttcgcagcag cggtttcttt accagactcg
       61 agtgcggccg caagcttgtc gacggagctc gaattcacgg gcaagggatt catcaacggt
      121 tacaggctta ttcaccggac tacagcctcc accagacctt ggaacaatgg cagaccatct
      181 tccccaccca tgagaggatc aaccagatct tctggatgag gcatcatgcc gcaaatgcgt
      241 ttgttttcgc tcagaatacc cgcaatgctg ttgatgctgc cattggggtt gctgatggca
      301 tcatcagcct gcacattgcc tttggcatcg gcatagtgga aagccacaag accttcacct
      361 tcaagccgtt ccagcgtggc gatatctgcc gtgtaattgc catcaccatg ggccagaggg
      421 gtgcggaaaa catcaccctt cttccacttg tgagtgaaag gtgtgttctc tgcttccacc
      481 ttcaggtagc aatcctgaga gagaaagcgc aggttggcgt tgcgcaacag agcaccgggc
      541 agaagccctg tttccgttaa aatctggaag ccgttgcaca cacccaaaat atggccaccc
      601 ttttgggcaa aggctttgat agccgccatg atgggagaat gcgctgccat ggcaccacag
      661 cgcagataat cgccatagct gaaaccacct ggcagcacga ctagatcggt atctcccaga
      721 tctgtttcat ggtgccagat catgcgcggg gcctgccctg taacgcgctt gagggcaatg
      781 gccatatcgc gctcccggtt tgtgccgggg aatacgataa ttgctgcttt catatgtata
      841 tctccttctt atacttaact aatatactaa gatggggaat tgttatccgc tcacaattcc
      901 cctatagtga gtcgtattaa tttcgattat gcggccgtgt acaatacgat tactttctgt
      961 tcgacttaag cattatgcgg cc
//
