LOCUS       sequence-141186-         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0 - SJM915 promoter (5' CTAT/3' GTAC fusion).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pBP-SJM915
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(19..62)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    65..84
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     primer_bind     complement(217..245)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 ggcgtatcac gaggcagaat ttcagataaa aaaaatcctt agctttcgct aaggatgatt
       61 tctggaattc gcggccgctt ctagaacgtc tcaatctggt ctcactattt tatggctagc
      121 tcagtccttg gtattatgct agcgtacaga gaccagatct tttaacttta agaaggagat
      181 atacatatgc gtaaaggaga agaacttttc actggagttg tcccaattct tgttgaatta
      241 gatggtgatg ttaatgggca caaattttct gtcagtggag agggtgaagg tgatgcaaca
      301 tacggaaaac ttacccttaa atttatttgc actactggaa aactacctgt tccatggcca
      361 acacttgtca ctactttcgg ttatggtgtt caatgctttg cgagataccc agatcatatg
      421 aaacagcatg actttttcaa gagtgccatg cccgaaggtt atgtacagga aagaactata
      481 tttttcaaag atgacgggaa ctacaagaca cgtgctgaag tcaagtttga aggtgatacc
      541 cttgttaata gaatcgagtt aaaaggtatt gattttaaag aagatggaaa cattcttgga
      601 cacaaattgg aatacaacta taactcacac aatgtataca tcatggcaga caaacaaaag
      661 aatggaatca aagttaactt caaaattaga cacaacattg aagatggaag cgttcaacta
      721 gcagaccatt atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt accagacaac
      781 cattacctgt ccacacaatc tgccctttcg aaagatccca acgaaaagag agatcacatg
      841 gtccttcttg agtt
//
