LOCUS       sequence-141190-         876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces formylglycinamide ribonucleotide amidotransferase, 
            N-terminal His6 tag (H6EcPurL).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Kappock
  TITLE     Purine biosynthesis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK556
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             35..52
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             56..76
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatataca tatgcatcat catcatcatc acggcgaaaa
       61 cctgtatttt cagggcggat ctatgatgga aattctgcgt ggttcgcctg cactgtcggc
      121 attccgaatc aacaaactgc tggcacgttt tcaggctgcc aggctcccgg ttcacaatat
      181 ttacgccgag tatgtccatt ttgctgacct caatgcgccg ttaaacgatg atgagcacgc
      241 acaacttgaa cgcctgctga aatatggtcc ggcactcgcc agccacgccc cgcaaggcaa
      301 actcctgctg gtgaccccgc gtcctggcac catctctccc tggtcttcga aagcgaccga
      361 tattgcccat aactgcgggc tacaacaggt aaaccgcctt gagcgcggcg ttgcttacta
      421 tatagaagcc ggtacgctga ccaatgaaca atggcagcag gttaccgctg aactgcacga
      481 ccgcatgatg gaaacggtct tttttgattt agatgatgca gagcagttgt ttgcccacca
      541 tcaaccgact ccggtcacaa gcgttgattt gctggggcag ggccgtcagg cgctgatcga
      601 cgctaacctg cgtcttggcc tggctctggc ggaagatgaa attgactatc tgcaggatgc
      661 tttcacaaag cttggtcgta acccgaacga catcgagctg tatatgttcg ctcaggcgaa
      721 ctctgagcac tgccgccaca aaatttttaa cgccgactgg attatcgacg gtgaacaaca
      781 gccgaaatcg ctgttcagga tgatcaaaaa tactttcgaa accacgccag atcacgttct
      841 ctctgcttat aaagataacg ccgccgtaat ggaagg
//
