LOCUS       sequence-141200-        2346 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  S.pombe recoded E2Crimson in universal C-terminal tagging plasmid, 
            nourseothricin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Al-Sady B, Greenstein RA, El-Samad HJ, Braun S, Madhani HD
  TITLE     Sensitive and Quantitative Three-Color Protein Imaging in Fission 
            Yeast Using Spectrally Diverse, Recoded Fluorescent Proteins with 
            Experimentally-Characterized In Vivo Maturation Kinetics.
  JOURNAL   PLoS One. 2016 Aug 1;11(8):e0159292. doi: 
            10.1371/journal.pone.0159292. eCollection 2016.
  PUBMED    27479698
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-08-1"; volume: "11(8):e0159292. doi"; pages: " 
            10.1371/journal.pone.0159292. eCollection 2016"
COMMENT     Sequence Label: BAS290 C terminal E2C natR
FEATURES             Location/Qualifiers
     source          1..2346
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     gene            1189..2308
                     /label=natMX6
                     /note="yeast selectable marker conferring nourseothricin 
                     resistance"
     promoter        1189..1532
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             1533..2105
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /label=NrsR
                     /note="confers resistance to nourseothricin"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGF
                     TLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNR
                     RLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFT
                     LCGLDTALYDGTASDGEQALYMSMPCP"
     terminator      2111..2308
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 ggtgctggat ctggttcatt aattaacatg gattctactg aaaacgttat taagcctttt
       61 atgcgtttta aggttcatat ggaaggttct gttaatggtc atgaatttga aattgaaggt
      121 gttggtgaag gtaagcctta tgaaggtact caaactgcta agcttcaagt tacaaaaggt
      181 ggtcctttac cttttgcttg ggatattctt tctcctcaat ttttttacgg ttctaaagct
      241 tacattaagc atcctgctga tattccagat taccttaagc aatcatttcc tgaaggtttt
      301 aagtgggaac gtgttatgaa ctttgaagat ggtggtgttg ttactgttac tcaagattct
      361 tcacttcaag atggtactct tatttaccat gttaagttta ttggtgttaa ttttccttct
      421 gatggtcctg ttatgcaaaa aaagacttta ggttgggaac cttctacaga acgtaattat
      481 cctcgtgatg gtgttcttaa gggtgaaaat catatggctc ttaagttgaa aggtggtggt
      541 cattaccttt gcgaatttaa gtctatttac atggctaaga agcctgttaa gcttcctggt
      601 tatcattacg ttgattacaa gcttgatatt acttctcata atgaagatta tactgttgtt
      661 gaacaatatg aacgtgctga agctcgtcat catctttttc aataaaaaaa gactaatgta
      721 aaattttttt ggttggttat tgaaaaagtc gatgccttgt ttgcgtttgt tttcctaggc
      781 gttttatgtc agaaggcatt tagaattagt atacaagtac tctttggtaa aattttatgt
      841 agcgactaaa atattaacta ttatagataa acaccttggg aataaaaagt aatttgctat
      901 agtaatttat taaacatgct cctacaacat taccacaatc ttttctcttg gattgacatt
      961 gaataagaaa agagtgaatt tttttagact tgtaatgata actatgtaca aagccaatga
     1021 aagatgtatg tagatgaatg taaaatacca tgtagacaaa caagataaaa cttggttata
     1081 aacattggtg ttggaacaga ataaattaga tgtcaaaaag tttcgtcaat atcacggcgc
     1141 gccagatctg tttagcttgc ctcgtccccg ccgggtcacc cggccagcga catggaggcc
     1201 cagaataccc tccttgacag tcttgacgtg cgcagctcag gggcatgatg tgactgtcgc
     1261 ccgtacattt agcccataca tccccatgta taatcatttg catccataca ttttgatggc
     1321 cgcacggcgc gaagcaaaaa ttacggctcc tcgctgcaga cctgcgagca gggaaacgct
     1381 cccctcacag acgcgttgaa ttgtccccac gccgcgcccc tgtagagaaa tataaaaggt
     1441 taggatttgc cactgaggtt cttctttcat atacttcctt tttaaatctt gctaggatac
     1501 agttctcaca tcacatccga acataaacaa ccatgggtac cactcttgac gacacggctt
     1561 accggtaccg caccagtgtc ccgggggacg ccgaggccat cgaggcactg gatgggtcct
     1621 tcaccaccga caccgtcttc cgcgtcaccg ccaccgggga cggcttcacc ctgcgggagg
     1681 tgccggtgga cccgcccctg accaaggtgt tccccgacga cgaatcggac gacgaatcgg
     1741 acgacgggga ggacggcgac ccggactctc ggacgttcgt cgcgtacggg gacgacggcg
     1801 acctggcggg cttcgtggtc gtctcgtact ccggctggaa ccgccggctg accgtcgagg
     1861 acatcgaggt cgccccggag caccgggggc acggggtcgg gcgcgcgttg atggggctcg
     1921 cgacggagtt cgcccgcgag cggggtgccg ggcacctctg gctggaggtc accaacgtca
     1981 acgcaccggc gatccacgcg taccggcgga tggggttcac cctctgcggc ctggacaccg
     2041 ccctgtacga cggcaccgcc tcggacggcg agcaggcgct ctacatgagc atgccctgcc
     2101 cctaatcagt actgacaata aaaagattct tgttttcaag aacttgtcat ttgtatagtt
     2161 tttttatatt gtagttgttc tattttaatc aaatgttagc gtgatttata ttttttttcg
     2221 cctcgacatc atctgcccag atgcgaagtt aagtgcgcag aaagtaatat catgcgtcaa
     2281 tcgtatgtga atgctggtcg ctatactgct gtcgattcga tactaacgcc gccatccagt
     2341 ttaaac
//
