LOCUS       sequence-141202-        2328 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  S.pombe recoded mKO2 in universal C-terminal tagging plasmid, 
            nourseothricin resistance..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Al-Sady B, Greenstein RA, El-Samad HJ, Braun S, Madhani HD
  TITLE     Sensitive and Quantitative Three-Color Protein Imaging in Fission 
            Yeast Using Spectrally Diverse, Recoded Fluorescent Proteins with 
            Experimentally-Characterized In Vivo Maturation Kinetics.
  JOURNAL   PLoS One. 2016 Aug 1;11(8):e0159292. doi: 
            10.1371/journal.pone.0159292. eCollection 2016.
  PUBMED    27479698
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-08-1"; volume: "11(8):e0159292. doi"; pages: " 
            10.1371/journal.pone.0159292. eCollection 2016"
COMMENT     Sequence Label: BAS288 C terminal mKO2 natR
FEATURES             Location/Qualifiers
     source          1..2328
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             28..678
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAH"
     gene            1171..2290
                     /label=natMX6
                     /note="yeast selectable marker conferring nourseothricin 
                     resistance"
     promoter        1171..1514
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             1515..2087
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /label=NrsR
                     /note="confers resistance to nourseothricin"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGF
                     TLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNR
                     RLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFT
                     LCGLDTALYDGTASDGEQALYMSMPCP"
     terminator      2093..2290
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 ggtgctggat ctggttcatt aattaacatg gtttctgtta ttaagcctga aatgaagatg
       61 cgttactata tggatggttc tgttaatggt catgaattta ctattgaagg tgaaggtact
      121 ggtcgtcctt atgaaggtca tcaagaaatg actcttcgtg ttactatggc tgaaggtggt
      181 cctatgcctt ttgcttttga tcttgtttct catgtttttt gctatggtca tcgtgttttt
      241 actaagtatc ctgaagaaat tcctgattac tttaagcaag cttttcctga aggtctttct
      301 tgggaacgtt ctcttgaatt tgaagatggt ggttctgctt ctgtttctgc tcatatttct
      361 cttcgtggta atacttttta ccataagtct aagtttactg gtgttaattt tcctgctgat
      421 ggtcctatta tgcaaaatca atctgttgat tgggaacctt ctactgaaaa gattactgct
      481 tctgatggtg ttcttaaggg tgatgttaca atgtacctta agttagaagg tggtggtaac
      541 cataagtgcc aaatgaagac tacttacaag gctgctaaag aaattcttga aatgcctggt
      601 gatcattaca ttggacatcg tcttgttcgt aagactgaag gtaacattac tgaacaagtt
      661 gaagatgctg ttgctcatta ttcttaaaaa aagactaatg taaaattttt ttggttggtt
      721 attgaaaaag tcgatgcctt gtttgcgttt gttttcctag gcgttttatg tcagaaggca
      781 tttagaatta gtatacaagt actctttggt aaaattttat gtagcgacta aaatattaac
      841 tattatagat aaacaccttg ggaataaaaa gtaatttgct atagtaattt attaaacatg
      901 ctcctacaac attaccacaa tcttttctct tggattgaca ttgaataaga aaagagtgaa
      961 tttttttaga cttgtaatga taactatgta caaagccaat gaaagatgta tgtagatgaa
     1021 tgtaaaatac catgtagaca aacaagataa aacttggtta taaacattgg tgttggaaca
     1081 gaataaatta gatgtcaaaa agtttcgtca atatcacggc gcgccagatc tgtttagctt
     1141 gcctcgtccc cgccgggtca cccggccagc gacatggagg cccagaatac cctccttgac
     1201 agtcttgacg tgcgcagctc aggggcatga tgtgactgtc gcccgtacat ttagcccata
     1261 catccccatg tataatcatt tgcatccata cattttgatg gccgcacggc gcgaagcaaa
     1321 aattacggct cctcgctgca gacctgcgag cagggaaacg ctcccctcac agacgcgttg
     1381 aattgtcccc acgccgcgcc cctgtagaga aatataaaag gttaggattt gccactgagg
     1441 ttcttctttc atatacttcc tttttaaatc ttgctaggat acagttctca catcacatcc
     1501 gaacataaac aaccatgggt accactcttg acgacacggc ttaccggtac cgcaccagtg
     1561 tcccggggga cgccgaggcc atcgaggcac tggatgggtc cttcaccacc gacaccgtct
     1621 tccgcgtcac cgccaccggg gacggcttca ccctgcggga ggtgccggtg gacccgcccc
     1681 tgaccaaggt gttccccgac gacgaatcgg acgacgaatc ggacgacggg gaggacggcg
     1741 acccggactc tcggacgttc gtcgcgtacg gggacgacgg cgacctggcg ggcttcgtgg
     1801 tcgtctcgta ctccggctgg aaccgccggc tgaccgtcga ggacatcgag gtcgccccgg
     1861 agcaccgggg gcacggggtc gggcgcgcgt tgatggggct cgcgacggag ttcgcccgcg
     1921 agcggggtgc cgggcacctc tggctggagg tcaccaacgt caacgcaccg gcgatccacg
     1981 cgtaccggcg gatggggttc accctctgcg gcctggacac cgccctgtac gacggcaccg
     2041 cctcggacgg cgagcaggcg ctctacatga gcatgccctg cccctaatca gtactgacaa
     2101 taaaaagatt cttgttttca agaacttgtc atttgtatag tttttttata ttgtagttgt
     2161 tctattttaa tcaaatgtta gcgtgattta tatttttttt cgcctcgaca tcatctgccc
     2221 agatgcgaag ttaagtgcgc agaaagtaat atcatgcgtc aatcgtatgt gaatgctggt
     2281 cgctatactg ctgtcgattc gatactaacg ccgccatcca gtttaaac
//
