LOCUS       sequence-141207-         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For retroviral expression of E3 ubiquitin ligase osTIR1 with N-term 
            NES and C-term 9x myc tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Holland AJ, Fachinetti D, Han JS, Cleveland DW
  TITLE     Inducible, reversible system for the rapid and complete degradation 
            of proteins in mammalian cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Dec 4;109(49):E3350-7. doi: 
            10.1073/pnas.1216880109. Epub 2012 Nov 12.
  PUBMED    23150568
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1216880109"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2012-12-4- 4"; volume: "109"; issue: "49"; pages: "E3350-7"
COMMENT     Sequence Label: pBabe TIR1-9myc
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      8..17
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 catcgccgcc accatggcat tacaaaaaaa attagaagaa ttagaattag atgaaggcgg
       61 cacgtacttc ccggaggagg tggtggagca catcttcagc ttcctgccgg cgcagcgcga
      121 ccgcaacacg gtctcgctcg tctgcaaggt gtggtacgag atcgagaggc tgagccgccg
      181 cggcgtcttc gtgggcaact gctacgccgt gcgcgccggc cgcgtcgccg cgcggttccc
      241 caacgtgcgg gcgctcacgg tgaaggggaa gccccacttc gccgacttca acctcgtgcc
      301 ccccgactgg ggcggctacg cggggccgtg gatcgaggcg gccgcgaggg gatgccacgg
      361 cctggaggag ctcaggatga agcggatggt ggtgtccgac gagagcctcg agctgctggc
      421 tcgctcgttc ccgcggttca gggctcttgt tcttatcagc tgcgaggggt tcagcactga
      481 cgggctagcc gccgtcgcga gccattgcaa gcttctgagg gagttggatt tgcaggaaaa
      541 tgaagtggag gatcgagggc ctaggtggct ttcctgcttc cctgattcct gcacatcact
      601 tgtctcattg aattttgcct gcatcaaagg ggaggttaat gctggttcac tggagagact
      661 tgttagcagg tccccaaacc tgcggagttt gaggctgaat cgatctgtat cggtagatac
      721 acttgcaaag atactactgc gtacccctaa cttggaggat ttggggacag ggaatttgac
      781 agatgacttc caaactgagt cctactttaa gcttaccagt gctctggaga aatgcaagat
      841 gttgaggagt ttgtctgg
//
