LOCUS       sequence-141208-         699 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For retroviral expression of E3 ubiquitin ligase osTIR1 with N-term 
            NES and C-term 9x myc tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Holland AJ, Fachinetti D, Han JS, Cleveland DW
  TITLE     Inducible, reversible system for the rapid and complete degradation 
            of proteins in mammalian cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Dec 4;109(49):E3350-7. doi: 
            10.1073/pnas.1216880109. Epub 2012 Nov 12.
  PUBMED    23150568
REFERENCE   2  (bases 1 to 699)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1216880109"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2012-12-4- 4"; volume: "109"; issue: "49"; pages: "E3350-7"
COMMENT     Sequence Label: pBabe TIR1-9myc
FEATURES             Location/Qualifiers
     source          1..699
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..64)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(71..100)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(107..136)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(155..184)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(191..220)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(227..256)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(275..304)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(311..340)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(347..376)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 atgatcgttc cactttttag ctagtggatc cgttcaagtc ttcttctgag attaattttt
       61 gttcaccgtt caagtcttcc tcggagatta gcttttgttc accgttcaaa tcttcttcag
      121 aaatcaactt ttgttcacct ctagaggatc cgttcaagtc ttcttctgag attaattttt
      181 gttcaccgtt caagtcttcc tcggagatta gcttttgttc accgttcaaa tcttcttcag
      241 aaatcaactt ttgttcacct ctagaggatc cgttcaagtc ttcttctgag attaattttt
      301 gttcaccgtt caagtcttcc tcggagatta gcttttgttc accgttcaaa tcttcttcag
      361 aaatcaactt ttgttcacca ctagcagcag aaccggagtc gactaggatt ttaacaaaat
      421 ttggtgcatc atccctcgcc ccagcagttg tgcggtacac atataatttc tccactttag
      481 gcagatctcc atggttttcc tccatttcat tgctaccatc ccgctcattt atgacctcaa
      541 cattgagcat cggcatcttt gacgcaagga cttggcaccc ctttaacgtg acattgcaag
      601 atgacatcca aagggatcgc attgtctcgt acctagcaaa attccccaag agtgcagcat
      661 caccaaacgg gctatctctt atctccagtt tccttaaat
//
