LOCUS       sequence-141210-         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  RBS_L5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pBP-TL12
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     8..25
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(49..106)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     terminator      complement(134..161)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(177..248)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(304..324)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 cgagcgcagc gagtcagtga gcgaggaagc ctgcataacg cgaagtaatc ttttcggttt
       61 taaagaaaaa gggcagggtg gtgacacctt gccctttttt gccggactgc agcgtctctg
      121 gcaaacatat aaacgcagaa aggcccaccc gaaggtgagc cagtgtgact ctagtagaga
      181 gcgttcaccg acaaacaaca gataaaacga aaggcccagt ctttcgactg agcctttcgt
      241 tttatttgat gcctggtcga ttatttgtat agttcatcca tgccatgtgt aatcccagca
      301 gctgttacaa actcaagaag gaccatgtga tctctctttt cgttgggatc tttcgaaagg
      361 gcagattgtg tggacaggta atggttgtct ggtaaaagga cagggccatc gccaattgga
      421 gtattttgtt gataatggtc tgctagttga acgcttccat cttcaatgtt gtgtctaatt
      481 ttgaagttaa ctttgattcc attcttttgt ttgtctgcca tgatgtatac attgtgtgag
      541 ttatagttgt attccaattt gtgtccaaga atgtttccat cttctttaaa atcaatacct
      601 tttaactcga ttctattaac aagggtatca ccttcaaact tgacttcagc acgtgtcttg
      661 tagttcccgt catctttgaa aaatatagtt ctttcctgta cataaccttc gggcatggca
      721 ctcttgaaaa agtcatgctg tttcatatga tctgggtatc tcgcaaagca ttgaacacca
      781 taaccgaaag tagtgacaag tgttggccat ggaacaggta gttttccagt agtgcaaata
      841 aatttaagg
//
