LOCUS       sequence-141213-         915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pAAV vector to express oG (optimized Glycoprotein) in a 
            Cre-dependent manner.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Kim EJ, Jacobs MW, Ito-Cole T, Callaway EM
  TITLE     Improved Monosynaptic Neural Circuit Tracing Using Engineered Rabies
            Virus Glycoproteins.
  JOURNAL   Cell Rep. 2016 Apr 26;15(4):692-699. doi: 
            10.1016/j.celrep.2016.03.067. Epub 2016 Apr 14.
  PUBMED    27149846
REFERENCE   2  (bases 1 to 915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2016.03.067"; journalName: "Cell Rep"; date: 
            "2016-04-26- 26"; volume: "15"; issue: "4"; pages: "692-699"
COMMENT     Sequence Label: pAAV-CAG-FLEX-oG-WPRE-SV40pA
FEATURES             Location/Qualifiers
     source          1..915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    66..99
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    170..203
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      223..232
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aattttgtaa tccagaggtt gattatcgat aagcttgata tcgaattcga acctcttcga
       61 gggacataac ttcgtataat gtatgctata cgaagttatg ggtcgatggt gagatctgga
      121 ctagagggtc gatggtgatg cttggatcaa aataggaaga atcccaatga taacttcgta
      181 taaagtatcc tatacgaagt tatactagaa ttcggtaccg ccgccaccat ggtcccacag
      241 gctctcctct tcgtccctct gctcgtcttc ccactctgct ttggtaaatt ccctatctac
      301 actatccccg acaagctggg cccctggagt cctatcgata ttcaccatct gtcatgccct
      361 aacaatctcg tggtcgagga cgaaggctgt accaacctga gcggattctc ctacatggag
      421 ctgaaagtgg gctatatctc tgctattaag atgaacgggt tcacatgcac tggtgtggtc
      481 accgaggcag aaacctacac aaattttgtg ggatatgtca ccacaacttt caagagaaaa
      541 cactttcggc caacaccaga cgcctgtcgg gcagcttaca actggaagat ggctggcgac
      601 ccccgctatg aggaaagcct gcacaatcct tacccagact atcattggct gcgcactgtg
      661 aagaccacaa aagagagcct ggtcatcatt tccccatctg tcgccgacct ggacccctac
      721 gaccgatctc tccacagtcc agtgtttccc ggcggaaact gctccggagt ggccgtcagc
      781 tccacttact gttccaccaa ccatgattat acaatctgga tgcctgagaa tccaaggctg
      841 gggatgtcct gcgacatctt caccaacagc aggggcaaga gggcctcaaa aggcagcgag
      901 acttgtggat ttgtg
//
