LOCUS       sequence-141214-         771 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tissue-specific knock-out in zebrafish, Cas9 and Cre driven by a UAS
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Di Donato V, De Santis F, Auer TO, Testa N, Sanchez-Iranzo H, 
            Mercader N, Concordet JP, Del Bene F
  TITLE     2C-Cas9: a versatile tool for clonal analysis of gene function.
  JOURNAL   Genome Res. 2016 Mar 8.
  PUBMED    26957310
REFERENCE   2  (bases 1 to 771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome Res.
            2016 Mar 8."
COMMENT     Sequence Label: pUAS:Cas9T2ACre;U6:sgRNA1;U6:sgRNA2
FEATURES             Location/Qualifiers
     source          1..771
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(210..229)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     264..283
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 gtcggagccg cgcgagatat ggcccgcgct ggagtttcaa taccggagat catgcaagct
       61 ggtggctgga ccaatgtaaa tattgtcatg aactatatcc gtaacctgga tagtgaaaca
      121 ggggcaatgg tgcgcctgct ggaagatggc gattagtcta gaactatagt gagtcgtatt
      181 acgtagatcc agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc
      241 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
      301 taagctgcaa taaaccttgt acaaagtggg ggatcctacg cttacagctc caaaactcta
      361 cggtaaacct acataaactg ctggttttca aattttaaag aatttaaggg tttacaggtt
      421 tactactaca cagtgattta ctgacacatg taagtgtaaa tgagttgaat aagtaagtaa
      481 gccatatacc acacatgaaa cacataccca gaagtcactg gtatatatag ccgtcctcca
      541 gactcccagc tcgagacgat tagatctaat gcgtctcgtt taagagctat gctggaaaca
      601 gcatagcaag tttaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg
      661 tgcttttttt caatgatgaa ttaattcgag ctccaccgcg gaagcttaaa caagaatctc
      721 tagttttctt tcttgctttt acttttactt ccttaatact caagtacaat t
//
