LOCUS       sequence-141217-         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pAAV vector to express oG (optimized Glycoprotein) in a 
            Flp-dependent manner.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Kim EJ, Jacobs MW, Ito-Cole T, Callaway EM
  TITLE     Improved Monosynaptic Neural Circuit Tracing Using Engineered Rabies
            Virus Glycoproteins.
  JOURNAL   Cell Rep. 2016 Apr 26;15(4):692-699. doi: 
            10.1016/j.celrep.2016.03.067. Epub 2016 Apr 14.
  PUBMED    27149846
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2016.03.067"; journalName: "Cell Rep"; date: 
            "2016-04-26- 26"; volume: "15"; issue: "4"; pages: "692-699"
COMMENT     Sequence Label: pAAV-CAG-fDIO-oG-WPRE-SV40pA
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    136..169
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     regulatory      185..194
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aattttgtaa tccagaggtt gattatcgat aagcttgata tcgaattctt cgaagttcct
       61 attcttcaaa aggtatagga acttctcttt gcaccattct aaagaataac agtgataatt
      121 tctgggttaa ggcaagaagt tcctattctt caaatagtat aggaacttct ctagtgctag
      181 cgccgccacc atggtcccac aggctctcct cttcgtccct ctgctcgtct tcccactctg
      241 ctttggtaaa ttccctatct acactatccc cgacaagctg ggcccctgga gtcctatcga
      301 tattcaccat ctgtcatgcc ctaacaatct cgtggtcgag gacgaaggct gtaccaacct
      361 gagcggattc tcctacatgg agctgaaagt gggctatatc tctgctatta agatgaacgg
      421 gttcacatgc actggtgtgg tcaccgaggc agaaacctac acaaattttg tgggatatgt
      481 caccacaact ttcaagagaa aacactttcg gccaacacca gacgcctgtc gggcagctta
      541 caactggaag atggctggcg acccccgcta tgaggaaagc ctgcacaatc cttacccaga
      601 ctatcattgg ctgcgcactg tgaagaccac aaaagagagc ctggtcatca tttccccatc
      661 tgtcgccgac ctggacccct acgaccgatc tctccacagt ccagtgtttc ccggcggaaa
      721 ctgctccgga gtggccgtca gctccactta ctgttccacc aaccatgatt atacaatctg
      781 gatgcctgag aatccaaggc tggggatgtc cctgcgacat cttcaccaac agcaggg
//
