LOCUS       sequence-141219-         901 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bicistronic expression of Magneto2.0 and mCherry in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Wheeler MA, Smith CJ, Ottolini M, Barker BS, Purohit AM, Grippo RM, 
            Gaykema RP, Spano AJ, Beenhakker MP, Kucenas S, Patel MK, Deppmann 
            CD, Guler AD
  TITLE     Genetically targeted magnetic control of the nervous system.
  JOURNAL   Nat Neurosci. 2016 Mar 7. doi: 10.1038/nn.4265.
  PUBMED    26950006
REFERENCE   2  (bases 1 to 901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2016 Mar 7. doi: 10.1038/nn.4265."
COMMENT     Sequence Label: pcDNA3.0-Magneto2.0-p2A-mCherry
FEATURES             Location/Qualifiers
     source          1..901
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     41..60
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        41..59
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      94..103
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ggctaactag agaacccact gcttactggc ttatcgaaat taatacgact cactataggg
       61 agacccaagc ttggtaccgg gtctagaggt accgccacca tggcagatcc tggtgatggc
      121 ccccgtgcag cgcctgggga tgtggctgag ccccctggag acgagagtgg cacttctggt
      181 ggggaggcct tccccctctc ttccctggcc aacctgtttg agggagagga aggctcctct
      241 tctctttcac cagtggatgc tagccgccct gctggccccg gggatggacg tccaaacctg
      301 cgtatgaagt tccagggcgc tttccgcaag ggggttccca accccattga cctgctggag
      361 tccaccctgt atgagtcctc agtagtgcct gggcccaaga aagcgcccat ggattcgttg
      421 ttcgactatg gcacttaccg gcaccacccc agtgacaaca agagatggag gaggaaggtc
      481 gtagagaagc agccacagag ccccaaagct cccgcccccc agccaccccc catcctcaaa
      541 gtcttcaacc ggcccatcct ctttgacatc gtgtcccggg gctccactgc cgacctggac
      601 ggactgctct cctacttgct gacccacaag aagcgcctga ctgatgagga gttccgggaa
      661 ccatccacag ggaagacctg cctgcccaag gcacttctga acttaagcaa tggccgaaac
      721 gacaccatcc cagtgttgct ggacattgcg gaacgcacgg gcaacatgcg ggagttcatc
      781 aactcgccct tcagagacat ctactaccga gggcagacgg cactgcacat cgccattgaa
      841 cggcgctgca agcattacgt ggagctcctg gtggcccagg gagccgatgt gcacgcgcag
      901 g
//
