LOCUS       sequence-141231-        1035 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of human APP fused to EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Currinn H, Guscott B, Balklava Z, Rothnie A, Wassmer T
  TITLE     APP controls the formation of PI(3,5)P vesicles through its binding 
            of the PIKfyve complex.
  JOURNAL   Cell Mol Life Sci. 2015 Jul 28.
  PUBMED    26216398
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Mol 
            Life Sci. 2015 Jul 28."
COMMENT     Sequence Label: pEGFP-n1-APP
FEATURES             Location/Qualifiers
     source          1..1035
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(401..412)
                     /codon_start=1
                     /gene="cspA"
                     /product="translation enhancing element for E. coli (Qing 
                     et al., 2004)"
                     /label=TEE
                     /translation="NHKV"
     CDS             892..912
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
ORIGIN
        1 gcagagctgg tttagtgaac cgtcagatcc gctagcgcta ccggactcag atctcgagct
       61 caagcttgcc accatgctgc ccggtttggc actgctcctg ctggccgcct ggacggctcg
      121 ggcgctggag gtacccactg atggtaatgc tggcctgctg gctgaacccc agattgccat
      181 gttctgtggc agactgaaca tgcacatgaa tgtccagaat gggaagtggg attcagatcc
      241 atcagggacc aaaacctgca ttgataccaa ggaaggcatc ctgcagtatt gccaagaagt
      301 ctaccctgaa ctgcagatca ccaatgtggt agaagccaac caaccagtga ccatccagaa
      361 ctggtgcaag cggggccgca agcagtgcaa gacccatccc cactttgtga ttccctaccg
      421 ctgcttagtt ggtgagtttg taagtgatgc ccttctcgtt cctgacaagt gcaaattctt
      481 acaccaggag aggatggatg tttgcgaaac tcatcttcac tggcacaccg tcgccaaaga
      541 gacatgcagt gagaagagta ccaacttgca tgactacggc atgttgctgc cctgcggaat
      601 tgacaagttc cgaggggtag agtttgtgtg ttgcccactg gctgaagaaa gtgacaatgt
      661 ggattctgct gatgcggagg aggatgactc ggatgtctgg tggggcggag cagacacaga
      721 ctatgcagat gggagtgaag acaaagtagt agaagtagca gaggaggaag aagtggctga
      781 ggtggaagaa gaagaagccg atgatgacga ggacgatgag gatggtgatg aggtagagga
      841 agaggctgag gaaccctacg aagaagccac agagagaacc accagcattg ccaccaccac
      901 caccaccacc acagagtctg tggaagaggt ggttcgagtt cctacaacag cagccagtac
      961 ccctgatgcc gttgacaagt atctcgagac acctggggat gagaatgaac atgcccattt
     1021 ccagaaagcc aaaga
//
