LOCUS       sequence-141232-         936 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of human APP fused to EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Currinn H, Guscott B, Balklava Z, Rothnie A, Wassmer T
  TITLE     APP controls the formation of PI(3,5)P vesicles through its binding 
            of the PIKfyve complex.
  JOURNAL   Cell Mol Life Sci. 2015 Jul 28.
  PUBMED    26216398
REFERENCE   2  (bases 1 to 936)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Mol 
            Life Sci. 2015 Jul 28."
COMMENT     Sequence Label: pEGFP-n1-APP
FEATURES             Location/Qualifiers
     source          1..936
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     208..229
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      271..280
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cttcgggcat ggcggacttg aagaagtcgt gctgcttcat gtggtcgggg tagcggctga
       61 agcactgcac gccgtaggtc agggtggtca cgagggtggg ccagggcacg ggcagcttgc
      121 cggtggtgca gatgaacttc agggtcagct tgccgtaggt ggcatcgccc tcgccctcgc
      181 cggacacgct gaacttgtgg ccgtttacgt cgccgtccag ctcgaccagg atgggcacca
      241 ccccggtgaa cagctcctcg cccttgctca ccatggtggc gaccggtgga tcccgggccc
      301 gcggtaccgt cgacccgttc tgcatctgct caaagaactt gtaggttgga ttttcgtagc
      361 cgttctgctg catcttggac aggtggcgct cctctggggt gacagcggcg tcaacctcca
      421 ccacaccatg atgaatggat gtgtactgtt tcttcttcag catcaccaag gtgatgacga
      481 tcactgtcgc tatgacaaca ccgcccacca tgagtccaat gattgcacct ttgtttgaac
      541 ccacatcttc tgcaaagaac accaattttt gatgatgaac ttcatatcct gagtcatgtc
      601 ggaattctgc atccatcttc acttcagaga tctcctccgt cttgatattt gtcaacccag
      661 aacctggtcg agtggtcagt cctcggtcgg cagcagggcg ggcatcaaca ggctcaactt
      721 cgttttctgt gttggctggc acagagtcag ccccaaaaga atgccacggc tggagatcgt
      781 ccaggctgaa ctctccattc acgggaagga gctccacggt ggttttcgtt tcggtcaaag
      841 atggcatgag agcatcgttt ccgtaactga tccttggttc actaatcatg ttggccaaga
      901 cgtcatctga atagttttgc tctttctgaa gcagct
//
