LOCUS       sequence-141235-        1038 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Bacillus subtilis formylglycinamidine ribonucleotide 
            synthetase glutaminase subunit, (PurQ).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Hoskins AA, Anand R, Ealick SE, Stubbe J
  TITLE     The formylglycinamide ribonucleotide amidotransferase complex from 
            Bacillus subtilis: metabolite-mediated complex formation.
  JOURNAL   Biochemistry. 2004 Aug 17;43(32):10314-27.
  PUBMED    15301530
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry."; date: "2004-08-17"; volume: "43(32)"; pages: 
            "10314-27"
COMMENT     Sequence Label: pJK044 (pET-PurQ-wt)
FEATURES             Location/Qualifiers
     source          1..1038
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..47)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             793..815
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    830..854
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(854..873)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(855..873)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(957..976)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 tttcgggctt tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc
       61 gcaagcttgt cgacggagct cgaattcgtg atttactcga gtcaagcagt agtgacatga
      121 gtttccctcc aatttttcac gatagactgg aacaatttaa gaccgtcggc gcttccaagc
      181 agttcatcga ccgcgcgctc agggtgaggc atcatgccta atacattgcc tttctcattc
      241 acgacaccgg caatgtcgct gacacttcca ttaatattag agccgtatgt gaaagcaatt
      301 tgattgtttt cctttaatgt agcaagcgtc tcgtcatcac agtagaaatt cccttcacca
      361 tgggcaaccg ggattgtaat cgattctccc ttttcgtagg aagctgtgaa taaggtttcg
      421 tcgttctgaa caatcaattc aaccggacgg caaatgaact tcagatcttt gttgcgtctc
      481 attgcgcctg gcagcagccc aagctcctgt aaaatctgga atccgttaca gacgccaaga
      541 acaggttttc cttcagccgc tgcttgtttg acagctggca taatattcgc aaatcgggcg
      601 atggcgccgc atcttaagta atcgccgtaa gaaaatcctc ccggaattaa cacgccgtcg
      661 aagccgtcaa ggcttgtttc ctcatgccag acgtattcca cttcatggcc gagctcatcc
      721 tttacagcat gatacatatc gatatcacag ttggagccgg gtaacacaat caccgcaaat
      781 ttcatatgta tatctccttc ttaaagttaa acaaaattat ttctagaggg gaattgttat
      841 ccgctcacaa ttcccctata gtgagtcgta ttaatttcgc gggatcgaga tctcgatcct
      901 ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta
      961 tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg
     1021 tttcggcgtg ggtatgga
//
