LOCUS       sequence-141243-        1005 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Bacillus subtilis formylglycinamidine ribonucleotide 
            synthetase glutaminase subunit, A128T mutant (PurQ-A128T) .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Hoskins AA, Anand R, Ealick SE, Stubbe J
  TITLE     The formylglycinamide ribonucleotide amidotransferase complex from 
            Bacillus subtilis: metabolite-mediated complex formation.
  JOURNAL   Biochemistry. 2004 Aug 17;43(32):10314-27.
  PUBMED    15301530
REFERENCE   2  (bases 1 to 1005)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry."; date: "2004-08-17"; volume: "43(32)"; pages: 
            "10314-27"
COMMENT     Sequence Label: pJK040 (pET-PurQ-A128T)
FEATURES             Location/Qualifiers
     source          1..1005
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(26..43)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             785..807
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    822..846
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(846..865)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(847..865)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(949..968)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gggctttgtt agcagccgga tctcagtggt ggtggtggtg gtgctcgagt gcggccgcaa
       61 gcttgtcgac ggagctcgaa ttcgtgattc gagtcaagca gtagtgacat gagtttccct
      121 ccaatttttc acgatagact ggaacaattt aagaccgtcg gcgcttccaa gcagttcatc
      181 gaccgcgcgc tcagggtgag gcatcatgcc taatacattg cctttctcat tcacgacacc
      241 ggcaatgtcg ctgacacttc cattaatatt agagccgtat gtgaaagcaa tttgattgtt
      301 ttcctttaat gtagcaagcg tctcgtcatc acagtagaaa ttcccttcac catgggcaac
      361 cgggattgta atcgattctc ccttttcgta ggaagttgtg aataaggttt cgtcgttctg
      421 aacaatcaat tcaaccggac ggcaaatgaa cttcagatct ttgttgcgtc tcattgcgcc
      481 tggcagcagc ccaagctcct gtaaaatctg gaatccgtta cagacgccaa gaacaggttt
      541 tccttcagcc gctgcttgtt tgacagctgg cataatattc gcaaatcggg cgatggcgcc
      601 gcatcttaag taatcgccgt aagaaaatcc tcccggaatt aacacgccgt cgaagccgtc
      661 aaggcttgtt tcctcatgcc agacgtattc cacttcatgg ccgagctcat cctttacagc
      721 atgatacata tcgatatcac agttggagcc gggtaacaca atcaccgcaa atttcatatg
      781 tatatctcct tcttaaagtt aaacaaaatt atttctagag gggaattgtt atccgctcac
      841 aattccccta tagtgagtcg tattaatttc gcgggatcga gatctcgatc ctctacgccg
      901 gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg
      961 acatcaccga tggggaagat cgggctcgcc acttcgggcc tcagg
//
