LOCUS       sequence-141245-         784 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MoClo Basic Part: RBS - Weiss RBS, high strength. Modified from 
            Bba_B0030 to adjust spacing in MC system. [B:B0030m:C].
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Densmore
  TITLE     B0030m_BC
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: B0030m_BC
FEATURES             Location/Qualifiers
     source          1..784
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     4..21
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    27..47
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     RBS             64..75
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     misc_feature    101..122
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      125..168
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(172..190)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        258..362
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(581..600)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cgcagcgagt cagtgagcga ggaagcctgc agcggccgct actagtaggt ctctcattta
       61 gtatttctcc tctttaatct ctagtatgag acccactagt ctctagaagc ggccgcgaat
      121 tccagaaatc atccttagcg aaagctaagg atttttttta tctgaaattc tgcctcgtga
      181 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
      241 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
      301 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
      361 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
      421 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
      481 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
      541 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
      601 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
      661 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
      721 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
      781 tcgg
//
