LOCUS       sequence-141251-         884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pAAV vector to express oG (optimized Glycoprotein) in a 
            Cre-dependent manner.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Kim EJ, Jacobs MW, Ito-Cole T, Callaway EM
  TITLE     Improved Monosynaptic Neural Circuit Tracing Using Engineered Rabies
            Virus Glycoproteins.
  JOURNAL   Cell Rep. 2016 Apr 26;15(4):692-699. doi: 
            10.1016/j.celrep.2016.03.067. Epub 2016 Apr 14.
  PUBMED    27149846
REFERENCE   2  (bases 1 to 884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2016.03.067"; journalName: "Cell Rep"; date: 
            "2016-04-26- 26"; volume: "15"; issue: "4"; pages: "692-699"
COMMENT     Sequence Label: pAAV-Ef1a-DIO-oG-WPRE-hGH
FEATURES             Location/Qualifiers
     source          1..884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    206..794
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             312..323
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     721..741
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     protein_bind    complement(819..852)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ttccacttgc cccttgctcc ataccacccc cctccacccc ataatattat agaaggacac
       61 ctagtcagac aaaatgatgc aacttaattt tattaggaca aggctggtgg gcactggagt
      121 ggcaacttcc agggccagga gaggcactgg ggaggggtca cagggatgcc acccgtagat
      181 ctctcgagca gcgctcggta tcgatgcggg gaggcggccc aaagggagat ccgactcgtc
      241 tgagggcgaa ggcgaagacg cggaagaggc cgcagagccg gcagcaggcc gcgggaagga
      301 aggtccgctg gattgagggc cgaagggacg tagcagaagg acgtcccgcg cagaatccag
      361 gtggcaacat aggcgagcag ccaaggaaag gacgatgatt tccccgacaa caccacggaa
      421 ttgtcagtgc ccaacagccg agcccctgtc cagcagcggg caaggcaggc ggcgatgagt
      481 tccgccgtgg caatagggag ggggaaagcg aaagtcccgg aaaggagctg acaggtggtg
      541 gcaatgcccc aaccagtggg ggttgcgtca gcaaacacag tgcacaccac gccacgttgc
      601 ctgacaacgg gccacaactc ctcataaaga gacagcaacc aggatttata caaggaggag
      661 aaaatgaaag ccatacggga agcaatagca tgatacaaag gcattaaagc agcgtatcca
      721 catagcgtaa aaggagcaac atagttaaga ataccagtca atctttcaca aattttgtaa
      781 tccagaggtt gattatcgat aagcttgata tcgaattcat aacttcgtat agcatacatt
      841 atacgaagtt attctttgca ccattctaaa gaataacagt gata
//
