LOCUS       sequence-141260-         757 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Calcium Sensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 757)
  TITLE     1.	AAV  Synapsin  Intron H2B Gcamp 6f wpre  Pzac2.1
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 757)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: AAV Cag Flex H2B Gcamp6s reverse
FEATURES             Location/Qualifiers
     source          1..757
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    3..36
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    99..132
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     700..719
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ccataacttc gtataaagta tcctatacga agttatatca aaataggaag accaatgctt
       61 caccatcgac ccgaattgcc aagcatcacc atcgacccat aacttcgtat aatgtatgct
      121 atacgaagtt atactaccgg tcacttcgct gtcatcattt gtacaaactc ttcgtagttt
      181 acctgaccat ccccatcgat gtctgcttcc ctgatcattt catcaacctc ttcatctgtt
      241 aacttctctc caaggtttgt catcacgtgg cgaagctctg ctgcactgat gtagccattg
      301 ccatccttat caaacacacc gaacgcttct ctaatttctt cttccgtgtc cctgtatttc
      361 atttttcttg ccatcattgt caggaactca gggaagtcga ttgtgccgtc accgtcggca
      421 tctacttcat tgatcatgtc ctgcagctct gcttctgtgg ggttctgccc cagagaccgc
      481 atcaccgtcc ccagctcctt ggttgttatt gtcccatccc cgtccttgtc aaatagggag
      541 aaagcctctt taaattctgc gatctgctct tcagtcagtt ggtccggcag gttgtactcc
      601 agcttgtgcc ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag ctcgatgcgg
      661 ttcaccaggg tgtcgccctc gaacttcacc tcggcgcggg tcttgtagtt gccgtcgtcc
      721 ttgaagaaga tggtgcgctc ctggatgtag ccttcgg
//
