LOCUS       sequence-141262-         804 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gene targeting vector for the mouse Rosa26 locus, including a CAG 
            promoter, loxP flanked stop cassette and GFP, for cloning into AscI.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Chu VT, Weber T, Graf R, Sommermann T, Petsch K, Sack U, Volchkov P,
            Rajewsky K, Kuhn R
  TITLE     Efficient generation of Rosa26 knock-in mice using CRISPR/Cas9 in 
            C57BL/6 zygotes.
  JOURNAL   BMC Biotechnol. 2016 Jan 16;16(1):4. doi: 10.1186/s12896-016-0234-4.
  PUBMED    26772810
REFERENCE   2  (bases 1 to 804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2016-01-16"; volume: "16(1):4. doi"; pages: " 
            10.1186/s12896-016-0234-4"
COMMENT     Sequence Label: pR26 CAG/GFP Asc
FEATURES             Location/Qualifiers
     source          1..804
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     29..48
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    complement(84..117)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    124..172
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     complement(290..309)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 gccttcttct ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat
       61 tttggcaaag aattcttaat taaataactt cgtatagcat acattatacg aagttatgga
      121 tccgaacaaa cgacccaaca cccgtgcgtt ttattctgtc tttttattgc cgatcccctc
      181 agaagaactc gtcaagaagg cgatagaagg cgatgcgctg cgaatcggga gcggcgatac
      241 cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag ctcttcagca atatcacggg
      301 tagccaacgc tatgtcctga tagcggtccg ccacacccag ccggccacag tcgatgaatc
      361 cagaaaagcg gccattttcc accatgatat tcggcaagca ggcatcgcca tgggtcacga
      421 cgagatcctc gccgtcgggc atgcgcgcct tgagcctggc gaacagttcg gctggcgcga
      481 gcccctgatg ctcttcgtcc agatcatcct gatcgacaag accggcttcc atccgagtac
      541 gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg
      601 tatgcagccg ccgcattgca tcagccatga tggatacttt ctcggcagga gcaaggtgag
      661 atgacaggag atcctgcccc ggcacttcgc ccaatagcag ccagtccctt cccgcttcag
      721 tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg
      781 ctgcctcgtc ctgcagttca ttca
//
