LOCUS       sequence-141263-         808 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Calcium sensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 808)
  TITLE     1.	AAV  Synapsin  Intron H2B Gcamp 6f wpre  Pzac2.1
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: AAV  Synapsin  Intron H2B Gcamp 6f wpre  Pzac2.1
FEATURES             Location/Qualifiers
     source          1..808
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      384..393
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             399..416
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             420..452
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(456..464,465..479)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     regulatory      486..495
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             507..563
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     776..797
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 agccagcgaa gtctgctccc gccccgaaaa agggctccaa gaaggcggtg actaaggcgc
       61 agaagaaagg cggcaagaag cgcaagcgca gccgcaagga gagctattcc atctatgtgt
      121 acaaggttct gaagcaggtc caccctgaca ccggcatttc gtccaaggcc atgggcatca
      181 tgaattcgtt tgtgaacgac attttcgagc gcatcgcagg tgaggcttcc cgcctggcgc
      241 attacaacaa gcgctcgacc atcacctcca gggagatcca gacggccgtg cgcctgctgc
      301 tgcctgggga gttggccaag cacgccgtgt ccgagggtac taaggccatc accaagtaca
      361 ccagcgctaa ggatccacca gtcgccacca tgggttctca tcatcatcat catcatggta
      421 tggctagcat gactggtgga cagcaaatgg gtcgggatct gtacgacgat gacgataagg
      481 atctcgccac catggtcgac tcatcacgtc gtaagtggaa taagacaggt cacgcagtca
      541 gagctatagg tcggctgagc tcactcgaga acgtctatat caaggccgac aagcagaaga
      601 acggcatcaa ggcgaacttc aagatccgcc acaacatcga ggacggcggc gtgcagctcg
      661 cctaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
      721 actacctgag cgtgcagtcc aaactttcga aagaccccaa cgagaagcgc gatcacatgg
      781 tcctgctgga gttcgtgacc gccgccgg
//
