LOCUS       sequence-141281-         871 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent reporter for single ribosome tracking.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Katz ZB, English BP, Lionnet T, Yoon YJ, Monnier N, Ovryn B, Bathe 
            M, Singer RH
  TITLE     Mapping translation 'hot-spots' in live cells by tracking single 
            molecules of mRNA and ribosomes.
  JOURNAL   Elife. 2016 Jan 13;5. pii: e10415. doi: 10.7554/eLife.10415.
  PUBMED    26760529
REFERENCE   2  (bases 1 to 871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2016-01-13"; volume: "5. pii: e10415. doi"; pages: " 
            10.7554/eLife.10415"
COMMENT     Sequence Label: p323-L10A-PATagRFP
FEATURES             Location/Qualifiers
     source          1..871
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(56..748)
                     /codon_start=1
                     /product="photoactivatable variant of the monomeric red 
                     fluorescent protein TagRFP (Subach et al., 2010)"
                     /label=PA-TagRFP
                     /note="mammalian codon-optimized"
                     /translation="ELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKVVE
                     GGPLPFAFDILATSFMYGSSTFINHTQGIPDFWKQSFPEGFTWERVTTYEDGGVLTATQ
                     DTSLQDGCLIYNVKIRGVNFPSNGPVMKKKTLGWEPSTEKLKPADGGLEGRVDMALKLV
                     GGGHLICNFKTTYRSKKPAKNLKMPGVYYVDRRLEIIKEADKETYWEQHEVAVARYSDL
                     PSKLGHKLN"
ORIGIN
        1 aattttgtaa tccagaggtt gattgtcgag tttacttgta cagctcgtcc atgccattaa
       61 gtttgtgccc cagtttgcta gggaggtcgg agtatctggc cacagccacc tcgtgctgct
      121 cccagtaggt ctctttgtcg gcctccttga tgatttccag tctgcggtcc acatagtaga
      181 cgccgggcat cttgaggttc ttagcgggtt tcttggatct gtatgtggtc ttgaagttgc
      241 agatcaggtg gcccccgccc acgagcttca gggccatgtc gaccctgcct tccaggccgc
      301 cgtcagcggg tttcagtttc tcggtgctcg gttcccagcc gagtgttttc ttcttcatca
      361 cagggccgtt ggatgggaag ttcacccctc tgatcttgac gttgtagatg aggcagccgt
      421 cctggaggct ggtgtcctgg gtagcggtca gcacgccccc gtcttcgtat gtggtgactc
      481 tctcccatgt gaagccctca gggaaggact gcttccagaa gtcggggatg ccctgggtgt
      541 ggttgatgaa ggtgctgctg ccgtacatga agctggtagc caggatgtcg aaggcgaagg
      601 ggagagggcc gccctcgacc accttgattc tcatggtctg ggtgccctcg tagggcttgc
      661 cttcgccctc ggatgtgcac ttgaagtggt ggttgttcac ggtgccctcc atgtacagct
      721 tcatgtgcat gttctcctta atcagctcct cgcccttgct cactcctcct cctcctcctc
      781 cggatccata cagacgctgg ggcttgccca tggtgctctt gatgtacaga gcccggacgt
      841 tctgccagtt tttcttgagt aaggacacca a
//
