LOCUS       sequence-141283-         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Calcium Sensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  TITLE     1.	AAV  Synapsin  Intron H2B Gcamp 6f wpre  Pzac2.1
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: AAV Cag Flex H2B Gcamp6f  reverse
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     31..50
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    85..118
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    181..214
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     783..802
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 atgccttctt ctttttccta cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc
       61 attttggcaa agaattggat ccccataact tcgtataaag tatcctatac gaagttatat
      121 caaaatagga agaccaatgc ttcaccatcg acccgaattg ccaagcatca ccatcgaccc
      181 ataacttcgt ataatgtatg ctatacgaag ttatactagc cggtcacttc gctgtcatca
      241 tttgtacaaa ctcttcgtag tttacctgac catccccatc gatgtctgct tccctgatca
      301 tttcatcaac ctcttcatct gttaacttct ctccaaggtt tgtcatcacg tggcgaagct
      361 ctgctgcact gatgtagcca ttgccatcct tatcaaacac accgaacgct tctctaattt
      421 cttcttccgt gtccctgtat ttcatttttc ttgccatcat tgtcaggaac tcagggaagt
      481 cgattgtgcc gtcaccgtcg gcatctactt cattgatcat gtcctgcagc tctgcttctg
      541 tggggttctg ccccagagac cgcatcaccg tccccagctc cttggttgtt attgtcccat
      601 ccccgtcctt gtcaaatagg gagaattcct ctttaaattc tgcgatctgc tcttcagtca
      661 gttggtccgg caggttgtac tccagcttgt gccccaggat gttgccgtcc tccttgaagt
      721 cgatgccctt cagctcgatg cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc
      781 gggtcttgta gttgccgtcg tccttgaaga
//
