LOCUS       sequence-141286-         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For gateway cloning Magneto2.0-p2A-mCherry into destination vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Wheeler MA, Smith CJ, Ottolini M, Barker BS, Purohit AM, Grippo RM, 
            Gaykema RP, Spano AJ, Beenhakker MP, Kucenas S, Patel MK, Deppmann 
            CD, Guler AD
  TITLE     Genetically targeted magnetic control of the nervous system.
  JOURNAL   Nat Neurosci. 2016 Mar 7. doi: 10.1038/nn.4265.
  PUBMED    26950006
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2016 Mar 7. doi: 10.1038/nn.4265."
COMMENT     Sequence Label: pCR8-Magneto2.0-p2A-mCherry
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(117..173)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(279..302)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(897..920)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ccctcgccct cgatctcgaa ctcgtggccg ttcacggagc cctccatgtg caccttgaag
       61 cgcatgaact ccttgatgat ggccatgtta tcctcctcgc ccttgctcac ggatccggga
      121 ccggggtttt cttccacgtc tcctgcttgc tttaacagag agaagttcgt ggcgccgctg
      181 cctgatccca cgttgatgtc gatctggtcc agggggatgt actcgccctc gctggtgatt
      241 ctgctcttgc tagccactcg ggaggagcct ccaccccctt tgtcatcgtc gtctttatag
      301 tcgctcccgc cacccccgcg tgcgctttca ttatcactgt ctcccagggt gtgcttgtca
      361 aagagatatt ccgccaagcc agattcgggc gctcccatct tgcgcaagtt ggtcacgtgg
      421 tcacccaatt ctttgatggc tttcacctgc tcattcaggt aatgtgtctc aatgaagtca
      481 cacaaatggg ggtcattttt gtcagtggcc agtttgtgca gttccagtag tgactgattc
      541 acattttttt ccaaatgtaa tgcacactcc attgcattca gcccgctctc ccagtcatca
      601 cagtctggtt tcttgatatc ctgaaggaag attcggccac ctcgttggtt ctgcagcttc
      661 atcagtttct cagcatgttc cctctcctca tgagattggt gaagaaagta tttggcaaag
      721 ttcttcaaag ccacatcatc gcggtcaaag tagtaagaca tggacaggta aacgtaggag
      781 gcgtagagct ccaggttgat ctggcggttg atggcggcct ctgagtcctg gtggtagttc
      841 tggcgcacct gcgaggtgga cgcggtcgtc atgacgcggg aggagccgcc gccgccttta
      901 tcatcatcat ctttataatc
//
