LOCUS       sequence-141287-         813 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expession of mCherry-LacR-BRCA1 1366-1437.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Orthwein A, Noordermeer SM, Wilson MD, Landry S, Enchev RI, Sherker 
            A, Munro M, Pinder J, Salsman J, Dellaire G, Xia B, Peter M, 
            Durocher D
  TITLE     A mechanism for the suppression of homologous recombination in G1 
            cells.
  JOURNAL   Nature. 2015 Dec 9. doi: 10.1038/nature16142.
  PUBMED    26649820
REFERENCE   2  (bases 1 to 813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Dec 9. doi: 10.1038/nature16142."
COMMENT     Sequence Label: pDEST-mCherry-LacR-BRCA1 1366-1437
FEATURES             Location/Qualifiers
     source          1..813
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             519..539
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    549..573
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cattaagttc tgtctcggcg cgtctgcgtc tggctggctg gcataaatat ctcactcgca
       61 atcaaattca gccgatagcg gaacgggaag gcgactggag tgccatgtcc ggttttcaac
      121 aaaccatgca aatgctgaat gagggcatcg ttcccactgc gatgctggtt gccaacgatc
      181 agatggcgct gggcgcaatg cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata
      241 tctcggtagt gggatacgac gataccgaag acagctcatg ttatatcccg ccgttaacca
      301 ccatcaaaca ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct
      361 ctcagggcca ggcggtgaag ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa
      421 ccaccctggc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
      481 agctggcacg acaggtttcc cgaagcagcc tgaggcctcc taagaagaag aggaaggttg
      541 gcgcgcccac aagtttgtac aaaaaagcag gcttcggtga agcagcatct gggtgtgaga
      601 gtgaaacaag cgtctctgaa gactgctcag ggctatcctc tcagagtgac attttaacca
      661 ctcagcagag ggataccatg caacataacc tgataaagct ccagcaggaa atggctgaac
      721 tagaagctgt gttagaacag catgggagcc agccttctaa cagctaccct agtataataa
      781 gtgactcttc tatagaccca gctttcttgt aca
//
