LOCUS       sequence-141298-         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  contains human U6 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Lonowski LA, Narimatsu Y, Riaz A, Delay CE, Yang Z, Niola F, Duda K,
            Ober EA, Clausen H, Wandall HH, Hansen SH, Bennett EP, Frodin M
  TITLE     Genome editing using FACS enrichment of nuclease-expressing cells 
            and indel detection by amplicon analysis.
  JOURNAL   Nat Protoc. 2017 Mar;12(3):581-603. doi: 10.1038/nprot.2016.165. 
            Epub 2017 Feb 16.
  PUBMED    28207001
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2016.165"; journalName: "Nat Protoc"; date: 
            "2017-03"; volume: "12"; issue: "3"; pages: "581-603"
COMMENT     Sequence Label: EPB104
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        24..272
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     24..44
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     195..214
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(417..434)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(607..626)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtccagagct cgatatcgaa ttcgagggcc tatttcccat gattccttca tatttgcata
       61 tacgatacaa ggctgttaga gagataattg gaattaattt gactgtaaac acaaagatat
      121 tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg gtagtttgca gttttaaaat
      181 tatgttttaa aatggactat catatgctta ccgtaacttg aaagtatttc gatttcttgg
      241 ctttatatat cttgtggaaa ggacgaaaca ccggaattcg atatcggtac ctggagcaca
      301 agactggcct catgggcctt ccgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
      361 cagctgcatt aacatggtca tagctgtttc cttgcgtatt gggcgctctc cgcttcctcg
      421 ctcactgact cgctgcgctc ggtcgttcgg gtaaagcctg gggtgcctaa tgagcaaaag
      481 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
      541 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
      601 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
      661 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
      721 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
      781 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
      841 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
//
