LOCUS       sequence-141299-         851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  contains human U6 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Lonowski LA, Narimatsu Y, Riaz A, Delay CE, Yang Z, Niola F, Duda K,
            Ober EA, Clausen H, Wandall HH, Hansen SH, Bennett EP, Frodin M
  TITLE     Genome editing using FACS enrichment of nuclease-expressing cells 
            and indel detection by amplicon analysis.
  JOURNAL   Nat Protoc. 2017 Mar;12(3):581-603. doi: 10.1038/nprot.2016.165. 
            Epub 2017 Feb 16.
  PUBMED    28207001
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2016.165"; journalName: "Nat Protoc"; date: 
            "2017-03"; volume: "12"; issue: "3"; pages: "581-603"
COMMENT     Sequence Label: EPB104
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(101..349)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(159..178)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(329..349)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(408..427)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(409..427)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(438..455)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(438..454)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(447..469)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc ggaaggccca
       61 tgaggccagt cttgtgctcc aggtaccgat atcgaattcc ggtgtttcgt cctttccaca
      121 agatatataa agccaagaaa tcgaaatact ttcaagttac ggtaagcata tgatagtcca
      181 ttttaaaaca taattttaaa actgcaaact acccaagaaa ttattacttt ctacgtcacg
      241 tattttgtac taatatcttt gtgtttacag tcaaattaat tccaattatc tctctaacag
      301 ccttgtatcg tatatgcaaa tatgaaggaa tcatgggaaa taggccctcg aattcgatat
      361 cgagctctgg acaagacacg tggccttgac ggccttccgc caattcgccc tatagtgagt
      421 cgtattacgt cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
      481 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
      541 gaggcccgca ccgaaacgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggagcg
      601 ccctgtagcg gccactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
      661 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
      721 acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc
      781 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
      841 ataaatgctt c
//
