LOCUS       sequence-141302-         976 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  gRNA scaffold with hU6 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Lonowski LA, Narimatsu Y, Riaz A, Delay CE, Yang Z, Niola F, Duda K,
            Ober EA, Clausen H, Wandall HH, Hansen SH, Bennett EP, Frodin M
  TITLE     Genome editing using FACS enrichment of nuclease-expressing cells 
            and indel detection by amplicon analysis.
  JOURNAL   Nat Protoc. 2017 Mar;12(3):581-603. doi: 10.1038/nprot.2016.165. 
            Epub 2017 Feb 16.
  PUBMED    28207001
REFERENCE   2  (bases 1 to 976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2016.165"; journalName: "Nat Protoc"; date: 
            "2017-03"; volume: "12"; issue: "3"; pages: "581-603"
COMMENT     Sequence Label: U6GRNA
FEATURES             Location/Qualifiers
     source          1..976
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(43..291)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(101..120)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(271..291)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     rep_origin      complement(353..941)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(433..452)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 acttgctatt tctagctcta aaacaggtct tctcgaagac ccggtgtttc gtcctttcca
       61 caagatatat aaagccaaga aatcgaaata ctttcaagtt acggtaagca tatgatagtc
      121 cattttaaaa cataatttta aaactgcaaa ctacccaaga aattattact ttctacgtca
      181 cgtattttgt actaatatct ttgtgtttac agtcaaatta attccaatta tctctctaac
      241 agccttgtat cgtatatgca aatatgaagg aatcatggga aataggccct cacatgtgag
      301 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
      361 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
      421 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
      481 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
      541 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
      601 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
      661 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
      721 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
      781 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
      841 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
      901 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
      961 ctacggggtc tgacgc
//
