LOCUS       sequence-141303-         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CbAkt promoter driven Cas9-2A-GFP (from px458).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Lonowski LA, Narimatsu Y, Riaz A, Delay CE, Yang Z, Niola F, Duda K,
            Ober EA, Clausen H, Wandall HH, Hansen SH, Bennett EP, Frodin M
  TITLE     Genome editing using FACS enrichment of nuclease-expressing cells 
            and indel detection by amplicon analysis.
  JOURNAL   Nat Protoc. 2017 Mar;12(3):581-603. doi: 10.1038/nprot.2016.165. 
            Epub 2017 Feb 16.
  PUBMED    28207001
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2016.165"; journalName: "Nat Protoc"; date: 
            "2017-03"; volume: "12"; issue: "3"; pages: "581-603"
COMMENT     Sequence Label: CAS9PBKS
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..33
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gataagatgg ccccaaagaa gaagcggaag gtcggtatcc acggagtccc agcagccgac
       61 aagaagtaca gcatcggcct ggacatcggc accaactctg tgggctgggc cgtgatcacc
      121 gacgagtaca aggtgcccag caagaaattc aaggtgctgg gcaacaccga ccggcacagc
      181 atcaagaaga acctgatcgg agccctgctg ttcgacagcg gcgaaacagc cgaggccacc
      241 cggctgaaga gaaccgccag aagaagatac accagacgga agaaccggat ctgctatctg
      301 caagagatct tcagcaacga gatggccaag gtggacgaca gcttcttcca cagactggaa
      361 gagtccttcc tggtggaaga ggataagaag cacgagcggc accccatctt cggcaacatc
      421 gtggacgagg tggcctacca cgagaagtac cccaccatct accacctgag aaagaaactg
      481 gtggacagca ccgacaaggc cgacctgcgg ctgatctatc tggccctggc ccacatgatc
      541 aagttccggg gccacttcct gatcgagggc gacctgaacc ccgacaacag cgacgtggac
      601 aagctgttca tccagctggt gcagacctac aaccagctgt tcgaggaaaa ccccatcaac
      661 gccagcggcg tggacgccaa ggccatcctg tctgccagac tgagcaagag cagacggctg
      721 gaaaatctga tcgcccagct gcccggcgag aagaagaatg gcctgttcgg aaacctgatt
      781 gccctgagcc tgggcctgac ccccaacttc aagagcaact tc
//
