LOCUS       sequence-141305-         686 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR synthetic transcription factor repressor plasmid encoding 
            dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for
            orthogonal repression of T7-lac promoter variant 3F2..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Cress BF, Jones JA, Kim DC, Leitz QD, Englaender JA, Collins SM, 
            Linhardt RJ, Koffas MA
  TITLE     Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible
            hybrid T7-lac promoters for modular, tuneable control of metabolic 
            pathway fluxes in Escherichia coli.
  JOURNAL   Nucleic Acids Res. 2016 Apr 13. pii: gkw231.
  PUBMED    27079979
REFERENCE   2  (bases 1 to 686)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Apr 13. pii: gkw231."
COMMENT     Sequence Label: pdCas9-M-3F2
FEATURES             Location/Qualifiers
     source          1..686
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggttccaa caagattatt ttataacttt tataacaaat aatcaaggag aaattcaaag
       61 aaatttatca gccataaaac aatacttaat actatagaat gataacaaaa taaactactt
      121 tttaaaagaa ttttgtgtta taatctattt attattaagt attgggtaat attttttgaa
      181 gagatatttt gaaaaagaaa aattaaagca tattaaacta atttcggagg tcattaaaac
      241 tattattgaa atcatcaaac tcattatgga tttaatttaa actttttatt ttaggaggca
      301 aaaatggata agaaatactc aataggctta gctatcggca caaatagcgt cggatgggcg
      361 gtgatcactg atgaatataa ggttccgtct aaaaagttca aggttctggg aaatacagac
      421 cgccacagta tcaaaaaaaa tcttataggg gctcttttat ttgacagtgg agagacagcg
      481 gaagcgactc gtctcaaacg gacagctcgt agaaggtata cacgtcggaa gaatcgtatt
      541 tgttatctac aggagatttt ttcaaatgag atggcgaaag tagatgatag tttctttcat
      601 cgacttgaag agtctttttt ggtggaagaa gacaagaagc atgaacgtca tcctattttt
      661 ggaaatatag tagatgaagt tgctta
//
