LOCUS       sequence-141308-         713 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR synthetic transcription factor repressor plasmid encoding 
            dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for
            orthogonal repression of T7-lac promoter variant 4F2..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Cress BF, Jones JA, Kim DC, Leitz QD, Englaender JA, Collins SM, 
            Linhardt RJ, Koffas MA
  TITLE     Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible
            hybrid T7-lac promoters for modular, tuneable control of metabolic 
            pathway fluxes in Escherichia coli.
  JOURNAL   Nucleic Acids Res. 2016 Apr 13. pii: gkw231.
  PUBMED    27079979
REFERENCE   2  (bases 1 to 713)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Apr 13. pii: gkw231."
COMMENT     Sequence Label: pdCas9-M-4F2
FEATURES             Location/Qualifiers
     source          1..713
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 acaagattat tttataactt ttataacaaa taatcaagga gaaattcaaa gaaatttatc
       61 agccataaaa caatacttaa tactatagaa tgataacaaa ataaactact ttttaaaaga
      121 attttgtgtt ataatctatt tattattaag tattgggtaa tattttttga agagatattt
      181 tgaaaaagaa aaattaaagc atattaaact aatttcggag gtcattaaaa ctattattga
      241 aatcatcaaa ctcattatgg atttaattta aactttttat tttaggaggc aaaaatggat
      301 aagaaatact caataggctt agctatcggc acaaatagcg tcggatgggc ggtgatcact
      361 gatgaatata aggttccgtc taaaaagttc aaggttctgg gaaatacaga ccgccacagt
      421 atcaaaaaaa atcttatagg ggctctttta tttgacagtg gagagacagc ggaagcgact
      481 cgtctcaaac ggacagctcg tagaaggtat acacgtcgga agaatcgtat ttgttatcta
      541 caggagattt tttcaaatga gatggcgaaa gtagatgata gtttctttca tcgacttgaa
      601 gagtcttttt tggtggaaga agacaagaag catgaacgtc atcctatttt tggaaatata
      661 gtagatgaag ttgcttatca tgagaaatat ccaactatct atcatctgcg aaa
//
