LOCUS       sequence-141310-         939 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EGFP Fluorescent reporter for overexpressed synaptophysin in 
            mammalian cell.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 939)
  AUTHORS   Harwell CC, Parker PR, Gee SM, Okada A, McConnell SK, Kreitzer AC, 
            Kriegstein AR
  TITLE     Sonic hedgehog expression in corticofugal projection neurons directs
            cortical microcircuit formation.
  JOURNAL   Neuron. 2012 Mar 22;73(6):1116-26. doi: 
            10.1016/j.neuron.2012.02.009. Epub 2012 Mar 21.
  PUBMED    22445340
REFERENCE   2  (bases 1 to 939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2012.02.009"; journalName: "Neuron"; date: 
            "2012-03-22- 22"; volume: "73"; issue: "6"; pages: "1116-26"
COMMENT     Sequence Label: CAG Double flox Synaptophysin-EGFP WPRE
FEATURES             Location/Qualifiers
     source          1..939
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(70..103)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(154..187)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      225..234
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gtcaatcttt cacaaatttt gtaatccaga ggttgattat cgataagctt gatttgggat
       61 atcgaattca taacttcgta tagcatacat tatacgaagt tattctttgc accattctaa
      121 agaataacag tgataatttc tgggttaagg caaataactt cgtataggat actttatacg
      181 aagttatgct agtaacggcc gccagtgtgc tggaattcgc ccttgccacc atggacgtgg
      241 tgaatcagct ggtggctggg ggtcagttcc gggtggtcaa ggagcccctt ggcttcgtga
      301 aggtgctgca gtgggtcttt gccatcttcg cctttgctac gtgtggcagc tacaccgggg
      361 agcttcggct gagcgtggag tgtgccaaca agacggagag tgccctcaac atcgaagttg
      421 aattcgagta ccccttcagg ctgcaccaag tgtactttga tgcaccctcc tgcgtcaaag
      481 ggggcactac caagatcttc ctggttgggg actactcctc gtcggctgaa ttctttgtca
      541 ccgtggctgt gtttgccttc ctctactcca tgggggccct ggccacctac atcttcctgc
      601 agaacaagta ccgagagaac aacaaagggc ctatgatgga ctttctggct acagccgtgt
      661 tcgctttcat gtggctagtt agttcatcag cctgggccaa aggcctgtcc gatgtgaaga
      721 tggccacgga cccagagaac attatcaagg agatgcccat gtgccgccag acagggaaca
      781 catgcaagga actgagggac cctgtgactt caggactcaa cacctcagtg gtgtttggct
      841 tcctgaacct ggtgctctgg gttggcaact tatggttcgt gttcaaggag acaggctggg
      901 cagccccatt catgcgcgca cctccaggcg ccccggaaa
//
