LOCUS       sequence-141313-        1092 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of N-terminal His-tagged.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Mullarky E, Lucki NC, Beheshti Zavareh R, Anglin JL, Gomes AP, 
            Nicolay BN, Wong JC, Christen S, Takahashi H, Singh PK, Blenis J, 
            Warren JD, Fendt SM, Asara JM, DeNicola GM, Lyssiotis CA, Lairson 
            LL, Cantley LC
  TITLE     Identification of a small molecule inhibitor of 3-phosphoglycerate 
            dehydrogenase to target serine biosynthesis in cancers.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Feb 16;113(7):1778-83. doi: 
            10.1073/pnas.1521548113. Epub 2016 Feb 1.
  PUBMED    26831078
REFERENCE   2  (bases 1 to 1092)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1521548113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-02-16- 16"; volume: "113"; issue: "7"; pages: "1778-83"
COMMENT     Sequence Label: pET28a-PHGDH
FEATURES             Location/Qualifiers
     source          1..1092
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(1..78)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     284..303
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     387..406
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        387..405
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    406..430
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             445..467
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             486..503
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             513..530
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt
       61 gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga
      121 agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca
      181 aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac
      241 aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat
      301 aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga
      361 ggatcgagat ctcgatcccg cgaaattaat acgactcact ataggggaat tgtgagcgga
      421 taacaattcc cctctagaaa taattttgtt taactttaag aaggagatat accatgggca
      481 gcagccatca tcatcatcat cacagcagcg gcctggtgcc gcgcggcagc catatgatgg
      541 cttttgcaaa tctgcggaaa gtgctcatca gtgacagcct ggacccttgc tgccggaaga
      601 tcttgcaaga tggagggctg caggtggtgg aaaagcagaa ccttagcaaa gaggagctga
      661 tagcggagct gcaggactgt gaaggcctta ttgttcgctc tgccaccaag gtgaccgctg
      721 atgtcatcaa cgcagctgag aaactccagg tggtgggcag ggctggcaca ggtgtggaca
      781 atgtggatct ggaggccgca acaaggaagg gcatcttggt tatgaacacc cccaatggga
      841 acagcctcag tgccgcagaa ctcacttgtg gaatgatcat gtgcctggcc aggcagattc
      901 cccaggcgac ggcttcgatg aaggacggca aatgggagcg gaagaagttc atgggaacag
      961 agctgaatgg aaagaccctg ggaattcttg gcctgggcag gattgggaga gaggtagcta
     1021 cccggatgca gtcctttggg atgaagacta tagggtatga ccccatcatt tccccagagg
     1081 tctcggcctc ct
//
