LOCUS       sequence-141315-         951 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pGRR NOR gate promoter responsive to gRNA-r11 and gRNA-r10. 
            Integrates in the URA locus of W303 and recovers URA function..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Gander MW, Vrana JD, Voje Jr WE, Carothers JM, Klavins E
  TITLE     Robust digital logic circuits in eukaryotic cells with CRISPR/dCas9 
            NOR gates
  JOURNAL   bioRxiv 041871
REFERENCE   2  (bases 1 to 951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            041871"
COMMENT     Sequence Label: pMOD6-pGRR-r11-r10-yeGFP
FEATURES             Location/Qualifiers
     source          1..951
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(33..57)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     916..932
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     916..932
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 ttttattatt aaataagtta taaaaaaaat aagtgtatac aaattttaaa gtgactctta
       61 ggttttaaaa cgaaaattct tattcttgag taactctttc ctgtaggtca ggttgctttc
      121 tcaggtatag catgaggtcg ctcttattga ccacacctcc cttaaccaga ttcgaaaagc
      181 ggcagtttat cattatcaat actcgccatt tcaaagaata cgtaaataat taatagtagt
      241 gattttccta actttattta gtcaaaaaat tagcctttta attctgctgt aacccgtaca
      301 tgcccaaaat agggggcggg ttacacagaa tatataacat cgtaggtgtc tgggtgaaca
      361 gtttattcct ggcatccact aaatataatg gagcccgctt tttaagctgg catccagaaa
      421 aaaaaagaat cccagcacca aaatattgtt ttcttcacca accatcagtt cataggtcca
      481 ttctcttagc gcaactacag agaacagggg cacaaacagg caaaaaacgg gcacaacctc
      541 aatggagtga tgcaacctgc ctggagtaaa tgatgacaca aggcaattga cccacgcatg
      601 tatctatctc attttcttac accttctatt accttctgct ctctctgatt tggaaaaagc
      661 tgaaaaaaaa ggttgaaacc agttccctga aattattccc ctacttgact aataagtaaa
      721 ttcctgcagc ccgggtactg tatgggcaaa gagacgcttg tcgtggcatg catgtgctct
      781 gtatgtatat aaaactcttg ttttcttctt ttctctaaat attctttcct gagtcgaatg
      841 tttatgtcac gcataaatta ctatacttct atagacacac aaacacaaat acacacacta
      901 atctagatat tggattctag aactagtgga tctacaaaat gtctaaaggt g
//
