LOCUS       sequence-141316-         981 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of N-terminal His-tagged.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Mullarky E, Lucki NC, Beheshti Zavareh R, Anglin JL, Gomes AP, 
            Nicolay BN, Wong JC, Christen S, Takahashi H, Singh PK, Blenis J, 
            Warren JD, Fendt SM, Asara JM, DeNicola GM, Lyssiotis CA, Lairson 
            LL, Cantley LC
  TITLE     Identification of a small molecule inhibitor of 3-phosphoglycerate 
            dehydrogenase to target serine biosynthesis in cancers.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Feb 16;113(7):1778-83. doi: 
            10.1073/pnas.1521548113. Epub 2016 Feb 1.
  PUBMED    26831078
REFERENCE   2  (bases 1 to 981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1521548113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-02-16- 16"; volume: "113"; issue: "7"; pages: "1778-83"
COMMENT     Sequence Label: pET28a-PHGDH
FEATURES             Location/Qualifiers
     source          1..981
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(101..148)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     144..162
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(215..232)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(282..314)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
ORIGIN
        1 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
       61 gcgtcccatt cgccaatccg gatatagttc ctcctttcag caaaaaaccc ctcaagaccc
      121 gtttagaggc cccaaggggt tatgctagtt attgctcagc ggtggcagca gccaactcag
      181 cttcctttcg ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg
      241 cggccgcaag cttgtcgacg gagctcgaat tcggatccgc gacccatttg ctgtccacca
      301 gtcatgctag ccatatgtta gaagtggaac tggaaggctt cagtcacatg ctgcttccac
      361 gcttccaggc tgggcagcaa ggaggagatg cccatgacgt gccaggtctc cccatctgac
      421 accagtgaag tctggtagga cagcagccgc acgcctgcct ctgccaggag gccaatcatg
      481 gtaggcagca ttgcagggtc agaggtctga gtccggaata ggagcagggg caggtccctg
      541 cggagaggca cttctggcct gaagacagct ccattgagcc cctgcagtac aggtgtagtg
      601 ccttggacca agcccacagc ctggtaaggg gcgcctgcca gggccacggc caggaggcat
      661 tccccgaagc cttgctcccc tggtgcagca gggctgtggg aggtggtgac attgaggcca
      721 gcctctttca ccagcagctt agcgttcacc aagttcacat ccgcctgctt ggaagcctct
      781 ttcaggaggc cgacaatgac tgcggggctt aggcagttcc cagcattctt cagggatgtt
      841 ccctgtgtta tcacctggat ggtccctttg ggggacccag cccaggctcg catcagtgtc
      901 cccagagctt ctgccagacc aatccaaggc ttggtgtgtg gagagaaggc actggtaagg
      961 gcctgggcat tcacaacccc c
//
