LOCUS       sequence-141317-         856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pGRR NOR gate promoter responsive to gRNA-r11 and gRNA-r10. 
            Integrates in the URA locus of W303 and recovers URA function..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Gander MW, Vrana JD, Voje Jr WE, Carothers JM, Klavins E
  TITLE     Robust digital logic circuits in eukaryotic cells with CRISPR/dCas9 
            NOR gates
  JOURNAL   bioRxiv 041871
REFERENCE   2  (bases 1 to 856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            041871"
COMMENT     Sequence Label: pMOD6-pGRR-r11-r10-yeGFP
FEATURES             Location/Qualifiers
     source          1..856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(687..703)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(717..735)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
ORIGIN
        1 tgtcccaatt ttggttgaat tagatggtga tgttaatggt cacaaatttt ctgtctccgg
       61 tgaaggtgaa ggtgatgcta cttacggtaa attgacctta aaatttattt gtactactgg
      121 taaattgcca gttccatggc caaccttagt cactactttc ggttatggtg ttcaatgttt
      181 tgcgagatac ccagatcata tgaaacaaca tgactttttc aagtctgcca tgccagaagg
      241 ttatgttcaa gaaagaacta tttttttcaa agatgacggt aactacaaga ccagagctga
      301 agtcaagttt gaaggtgata ccttagttaa tagaatcgaa ttaaaaggta ttgattttaa
      361 agaagatggt aacattttag gtcacaaatt ggaatacaac tataactctc acaatgttta
      421 catcatggct gacaaacaaa agaatggtat caaagttaac ttcaaaatta gacacaacat
      481 tgaagatggt tctgttcaat tagctgacca ttatcaacaa aatactccaa ttggtgatgg
      541 tccagtcttg ttaccagaca accattactt atccactcaa tctgccttat ccaaagatcc
      601 aaacgaaaag agagaccaca tggtcttgtt agaatttgtt actgctgctg gtattaccca
      661 tggtatggat gaattgtaca aataatgata ccgtcgacct cgagtcaatt agttatgtca
      721 cgcttacatt cacgccctcc ccccacatcc gctctaaccg aaaaggaagg agttagacaa
      781 cctgaagtct aggtccctat ttattttttt atagttatgt tagtattaag aacgttattt
      841 atatttcaaa tttttc
//
